Multi-spectral confocal scanning laser ophthalmoscope for retinal vessel oximetry

Arthur Lompado, Matthew H. Smith, Lloyd W. Hillman, Kurt R. Denninghoff

Research output: Contribution to journalConference articlepeer-review

14 Scopus citations


Scanning laser microscopy is a widely used technique in ophthalmoscopy for providing high-resolution real time images of the retina. We describe a scanning laser ophthalmoscope that acquires retinal images at four wavelengths for the purpose of measuring the oxygen saturation of blood in retinal arteries and veins. Images at all four wavelengths are obtained across a single video frame using a temporal interlacing technique. An extraction procedure then permits analysis of four monochromatic images. A technique for calculating oxygen saturation from a multi-spectral image set is presented, along with preliminary measurements. The choice of wavelengths dramatically affects the oxygen saturation calculation accuracy and we present an optimized wavelength set and the calculated oxygen saturation results. The potential applications for this technology range from the diagnosis of various ophthalmic diseases to the detection of blood loss in trauma victims.

Original languageEnglish (US)
Pages (from-to)67-73
Number of pages7
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 2000
EventSpectral Imaging: Instrumentation, Applications, and Analysis - San Jose, CA, USA
Duration: Jan 25 2000Jan 25 2000

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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