Multi-parametric analysis of cellular and subcellular function using spectral imaging

Ronald M. Lynch; Kevin D. Nullmeyer; Bridget K. Ford; Linda S. Tompkins; Vicki L. Sutherland; Michael R. Descour

Multi-parametric analysis of cellular and subcellular function using spectral imaging

Ronald M. Lynch, Kevin D. Nullmeyer, Bridget K. Ford, Linda S. Tompkins, Vicki L. Sutherland, Michael R. Descour

Research output: Contribution to journal › Conference article › peer-review

Abstract

The development of fluorescent probes that sense cellular constituents has revolutionized studies of cell physiology. To take full advantage of these probes for physiological studies, techniques that simultaneously monitor signal from multiple probes with high temporal resolution are required. This article discusses the use of spectral imaging approaches for simultaneous analysis of pH and Ca²⁺ in singlet cells.

Original language	English (US)
Pages (from-to)	79-87
Number of pages	9
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	3924
State	Published - 2000
Event	Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation - San Jose, CA, USA Duration: Jan 23 2000 → Jan 24 2000

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Cite this

@article{413b968d6afc468d9071adf04d1b8bb7,

title = "Multi-parametric analysis of cellular and subcellular function using spectral imaging",

abstract = "The development of fluorescent probes that sense cellular constituents has revolutionized studies of cell physiology. To take full advantage of these probes for physiological studies, techniques that simultaneously monitor signal from multiple probes with high temporal resolution are required. This article discusses the use of spectral imaging approaches for simultaneous analysis of pH and Ca2+ in singlet cells.",

author = "Lynch, {Ronald M.} and Nullmeyer, {Kevin D.} and Ford, {Bridget K.} and Tompkins, {Linda S.} and Sutherland, {Vicki L.} and Descour, {Michael R.}",

year = "2000",

language = "English (US)",

volume = "3924",

pages = "79--87",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation ; Conference date: 23-01-2000 Through 24-01-2000",

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TY - JOUR

T1 - Multi-parametric analysis of cellular and subcellular function using spectral imaging

AU - Lynch, Ronald M.

AU - Nullmeyer, Kevin D.

AU - Ford, Bridget K.

AU - Tompkins, Linda S.

AU - Sutherland, Vicki L.

AU - Descour, Michael R.

PY - 2000

Y1 - 2000

N2 - The development of fluorescent probes that sense cellular constituents has revolutionized studies of cell physiology. To take full advantage of these probes for physiological studies, techniques that simultaneously monitor signal from multiple probes with high temporal resolution are required. This article discusses the use of spectral imaging approaches for simultaneous analysis of pH and Ca2+ in singlet cells.

AB - The development of fluorescent probes that sense cellular constituents has revolutionized studies of cell physiology. To take full advantage of these probes for physiological studies, techniques that simultaneously monitor signal from multiple probes with high temporal resolution are required. This article discusses the use of spectral imaging approaches for simultaneous analysis of pH and Ca2+ in singlet cells.

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UR - http://www.scopus.com/inward/citedby.url?scp=0033721483&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:0033721483

SN - 0277-786X

VL - 3924

SP - 79

EP - 87

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation

Y2 - 23 January 2000 through 24 January 2000

ER -

Multi-parametric analysis of cellular and subcellular function using spectral imaging

Abstract

ASJC Scopus subject areas

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