Multi-modal contrast of tissue anatomy enables correlative biomarker imaging

Karl Garsha, Franklin Ventura, Gary Pestano, Michael Otter, Dea Nagy, Ray B. Nagle, Esteban Roberts, Michael Barnes

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Optical imaging techniques are being developed that promise to increase the information content related to specific molecular reporters. Such modalities do not produce contrast in the structural context of the surrounding tissue, making it difficult to reconcile molecular information with morphological context. We report a solution that enables visualization of the tissue morphology on formalin-fixed, paraffin embedded sections prepared for analytical biomarker imaging. Our approach combines modes of transmitted darkfield and fluorescence contrast and computer visualization to produce 2-component image data analogous to the classical hematoxylin and eosin histological stain. An interferometric hyperspectral image capture mode enables measurement of multiplexed biomarkers in annotated anatomic regions. The system enables practical correlative analysis of molecular changes within areas of anatomic pathology.

Original languageEnglish (US)
Title of host publicationMultimodal Biomedical Imaging X
EditorsFred S. Azar, Xavier Intes
ISBN (Electronic)9781628414066
StatePublished - 2015
EventMultimodal Biomedical Imaging X - San Francisco, United States
Duration: Feb 7 2015 → …

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherMultimodal Biomedical Imaging X
Country/TerritoryUnited States
CitySan Francisco
Period2/7/15 → …


  • Fluorescence
  • Histology
  • Hyperspectral
  • Microscopy
  • Molecular
  • Multilplex
  • Pathology
  • Q-dot
  • Quantitative
  • Quantum dot

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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