Multi-color fluorescence detection of ribosomal RNA in micro-channels

Michal Balberg, Krassimira Hristova, Margit Mau, Dominic Frigon, Henry C. Zeringue, David J. Brady, David J. Beebe, Lutgarde Raskin

Research output: Contribution to journalConference articlepeer-review

1 Scopus citations


A micro fluidic device capable of detecting the abundance of bacteria in an environmental solution is described. The micro channels are made of poly(dimethylsioxane) (PDMS) elastomer integrated with fused silica capillaries coated with Aluminum. The detection of specific bacteria is based on molecular probes (beacons) that emit a fluorescent signal only when hybridized to the target. This method allows hybridization in solution, without immobilization, and avoids washing of the unbound probes. By marking 16S rDNA oligonucleotide probes (different genetic sequences) with different color dyes, and detecting the spectral intensity of light in the micro-channel, different microorganisms can be detected in one sample. Miniaturization of the analytic device allows the use of small quantities of RNA molecules, as target molecules, and improves the detection limits. Future devices should incorporate a parallel array of micro-channels, and enable fast and parallel processing of the molecular signals.

Original languageEnglish (US)
Pages (from-to)35-40
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 2000
Externally publishedYes
EventMicro- and Nanotechnology for Biomedical and Environmental Applications - San Jose, CA, USA
Duration: Jan 26 2000Jan 27 2000

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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