Mouse rDNA nontranscribed spacer sequences are found flanking immunoglobulin CH genes and elsewhere throughout the genome

Norman Arnheim; Peter Seperack; Julian Banerji; Rhonda B. Lang; Roger Miesfeld; Kenneth B. Marcu

doi:10.1016/0092-8674(80)90166-X

Mouse rDNA nontranscribed spacer sequences are found flanking immunoglobulin C_H genes and elsewhere throughout the genome

Norman Arnheim
, Peter Seperack
, Julian Banerji
, Rhonda B. Lang
, Roger Miesfeld
, Kenneth B. Marcu

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

When a cloned 6 kb Eco RI-Sal I fragment of mouse ribosomal gene nontranscribed spacer DNA (rDNA NTS) was used to screen a BALB/c mouse gene library, 25% of the recombinant phage hybridized with it. In situ hybridization experiments and characterization of 12 clones selected using this probe supported the idea that sequences homologous to this rDNA NTS region are scattered throughout the genome. Subsequently, sequences homologous to mouse rDNA NTS were found flanking mouse μ, α and γ2b immunoglobulin C_H genes. One region was localized 3′ to the μ coding sequence, an area which has been identified as an intervening sequence between the secreted C_μ heavy chain terminus and the C terminal portion of the membrane-bound C_μ heavy chain.

Original language	English (US)
Pages (from-to)	179-185
Number of pages	7
Journal	Cell
Volume	22
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(80)90166-X
State	Published - 1980
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(80)90166-X

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@article{41c3be0b58b2442d8cd198f2794ae17d,

title = "Mouse rDNA nontranscribed spacer sequences are found flanking immunoglobulin CH genes and elsewhere throughout the genome",

abstract = "When a cloned 6 kb Eco RI-Sal I fragment of mouse ribosomal gene nontranscribed spacer DNA (rDNA NTS) was used to screen a BALB/c mouse gene library, 25\% of the recombinant phage hybridized with it. In situ hybridization experiments and characterization of 12 clones selected using this probe supported the idea that sequences homologous to this rDNA NTS region are scattered throughout the genome. Subsequently, sequences homologous to mouse rDNA NTS were found flanking mouse μ, α and γ2b immunoglobulin CH genes. One region was localized 3′ to the μ coding sequence, an area which has been identified as an intervening sequence between the secreted Cμ heavy chain terminus and the C terminal portion of the membrane-bound Cμ heavy chain.",

author = "Norman Arnheim and Peter Seperack and Julian Banerji and Lang, \{Rhonda B.\} and Roger Miesfeld and Marcu, \{Kenneth B.\}",

note = "Funding Information: We wish to thank Drs. P. Early, M. Davis and L. Hood for communicating results prior to publication. We thank L. Hood for sending us his mouse sperm gene library. We also wish to acknowledge the technical assistance of Susan Clash and Victoria Weiner. The advice and hospitality of Drs. Peter Rae and Joe Gall are also greatly appreciated. This work was supported by NIH grants to N. A. and K. B. M.",

year = "1980",

doi = "10.1016/0092-8674(80)90166-X",

language = "English (US)",

volume = "22",

pages = "179--185",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Mouse rDNA nontranscribed spacer sequences are found flanking immunoglobulin CH genes and elsewhere throughout the genome

AU - Arnheim, Norman

AU - Seperack, Peter

AU - Banerji, Julian

AU - Lang, Rhonda B.

AU - Miesfeld, Roger

AU - Marcu, Kenneth B.

N1 - Funding Information: We wish to thank Drs. P. Early, M. Davis and L. Hood for communicating results prior to publication. We thank L. Hood for sending us his mouse sperm gene library. We also wish to acknowledge the technical assistance of Susan Clash and Victoria Weiner. The advice and hospitality of Drs. Peter Rae and Joe Gall are also greatly appreciated. This work was supported by NIH grants to N. A. and K. B. M.

PY - 1980

Y1 - 1980

N2 - When a cloned 6 kb Eco RI-Sal I fragment of mouse ribosomal gene nontranscribed spacer DNA (rDNA NTS) was used to screen a BALB/c mouse gene library, 25% of the recombinant phage hybridized with it. In situ hybridization experiments and characterization of 12 clones selected using this probe supported the idea that sequences homologous to this rDNA NTS region are scattered throughout the genome. Subsequently, sequences homologous to mouse rDNA NTS were found flanking mouse μ, α and γ2b immunoglobulin CH genes. One region was localized 3′ to the μ coding sequence, an area which has been identified as an intervening sequence between the secreted Cμ heavy chain terminus and the C terminal portion of the membrane-bound Cμ heavy chain.

AB - When a cloned 6 kb Eco RI-Sal I fragment of mouse ribosomal gene nontranscribed spacer DNA (rDNA NTS) was used to screen a BALB/c mouse gene library, 25% of the recombinant phage hybridized with it. In situ hybridization experiments and characterization of 12 clones selected using this probe supported the idea that sequences homologous to this rDNA NTS region are scattered throughout the genome. Subsequently, sequences homologous to mouse rDNA NTS were found flanking mouse μ, α and γ2b immunoglobulin CH genes. One region was localized 3′ to the μ coding sequence, an area which has been identified as an intervening sequence between the secreted Cμ heavy chain terminus and the C terminal portion of the membrane-bound Cμ heavy chain.

UR - https://www.scopus.com/pages/publications/0019167448

UR - https://www.scopus.com/pages/publications/0019167448#tab=citedBy

U2 - 10.1016/0092-8674(80)90166-X

DO - 10.1016/0092-8674(80)90166-X

M3 - Article

C2 - 6775816

AN - SCOPUS:0019167448

SN - 0092-8674

VL - 22

SP - 179

EP - 185

JO - Cell

JF - Cell

IS - 1

ER -

Mouse rDNA nontranscribed spacer sequences are found flanking immunoglobulin C_H genes and elsewhere throughout the genome

Abstract

ASJC Scopus subject areas

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