Mouse- and human-derived primary gastric epithelial monolayer culture for the study of regeneration

Emma Teal, Nina G. Steele, Jayati Chakrabarti, Loryn Holokai, Yana Zavros

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

In vitro studies of gastric wound repair typically involves the use of gastric cancer cell lines in a scratch-wound assay of cellular proliferation and migration. One critical limitation of such assays, however, is their homogenous assortment of cellular types. Repair is a complex process which demands the interaction of several cell types. Therefore, to study a culture devoid of cellular subtypes, is a concern that must be overcome if we are to understand the repair process. The gastric organoid model may alleviate this issue whereby the heterogeneous collection of cell types closely reflects that of the gastric epithelium or other native tissues in vivo. Demonstrated here is a novel, in vitro scratch-wound assay derived from human or mouse 3-dimensional organoids which can then be transferred to a gastric epithelial monolayer as either intact organoids or as a single cell suspension of dissociated organoids. The goal of the protocol is to establish organoid-derived gastric epithelial monolayers that can be used in a novel scratch-wound assay system to study gastric regeneration.

Original languageEnglish (US)
Article numbere57435
JournalJournal of Visualized Experiments
Volume2018
Issue number135
DOIs
StatePublished - May 7 2018
Externally publishedYes

Keywords

  • Epithelial monolayer
  • Gastric organoids
  • Immunology and Infection
  • Issue 135
  • Regeneration
  • Repair
  • Scratch-wound assay
  • Stem cells

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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