Mouse adrenal chromaffin cell isolation

Aaron Kolski-Andreaco; Haijiang Cai; D. Spencer Currle; K. George Chandy; Robert H. Chow

doi:10.3791/129

Mouse adrenal chromaffin cell isolation

Aaron Kolski-Andreaco, Haijiang Cai, D. Spencer Currle, K. George Chandy, Robert H. Chow

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting. This protocol illustrates the method used to dissect the adrenals and then isolate the medullary region by stripping away the adrenal cortex. The digestion of the medulla into single chromaffin cells is then demonstrated.

Original language	English (US)
Article number	e129
Journal	Journal of Visualized Experiments
Issue number	2
DOIs	https://doi.org/10.3791/129
State	Published - Dec 2006
Externally published	Yes

Keywords

Adrenal
Developmental biology
Issue 2
Mouse
Neuroscience

ASJC Scopus subject areas

General Neuroscience
General Chemical Engineering
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.3791/129

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title = "Mouse adrenal chromaffin cell isolation",

abstract = "Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting. This protocol illustrates the method used to dissect the adrenals and then isolate the medullary region by stripping away the adrenal cortex. The digestion of the medulla into single chromaffin cells is then demonstrated.",

keywords = "Adrenal, Developmental biology, Issue 2, Mouse, Neuroscience",

author = "Aaron Kolski-Andreaco and Haijiang Cai and {Spencer Currle}, D. and {George Chandy}, K. and Chow, {Robert H.}",

year = "2006",

month = dec,

doi = "10.3791/129",

language = "English (US)",

journal = "Journal of Visualized Experiments",

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TY - JOUR

T1 - Mouse adrenal chromaffin cell isolation

AU - Kolski-Andreaco, Aaron

AU - Cai, Haijiang

AU - Spencer Currle, D.

AU - George Chandy, K.

AU - Chow, Robert H.

PY - 2006/12

Y1 - 2006/12

N2 - Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting. This protocol illustrates the method used to dissect the adrenals and then isolate the medullary region by stripping away the adrenal cortex. The digestion of the medulla into single chromaffin cells is then demonstrated.

AB - Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting. This protocol illustrates the method used to dissect the adrenals and then isolate the medullary region by stripping away the adrenal cortex. The digestion of the medulla into single chromaffin cells is then demonstrated.

KW - Adrenal

KW - Developmental biology

KW - Issue 2

KW - Mouse

KW - Neuroscience

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U2 - 10.3791/129

DO - 10.3791/129

M3 - Article

AN - SCOPUS:80355140367

SN - 1940-087X

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

IS - 2

M1 - e129

ER -

Mouse adrenal chromaffin cell isolation

Abstract

Keywords

ASJC Scopus subject areas

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