TY - JOUR
T1 - Morphological and molecular characterisation of Steinernema hermaphroditum n. sp. (Nematoda
T2 - Steinernematidae), an entomopathogenic nematode from Indonesia, and its phylogenetic relationships with other members of the genus
AU - Stock, S. Patricia
AU - Griffin, Christine T.
AU - Chaerani, Rani
N1 - Funding Information:
This work was supported in part by a USDA-Hatch-ARZT-136816-H-05-124 grant to S.P. Stock.
PY - 2004
Y1 - 2004
N2 - Steinernema hermaphroditum n. sp., a new entomopathogenic nematode isolated from soil samples in the Moluccan islands, Indonesia, is described. Morphological observations as well as biological evidence (cross-hybridisation studies) indicate the distinctness of S. hermaphroditum n. sp. from other Steinernema spp. This new species is characterised by the presence of hermaphrodites in the first adult generation. Key morphological diagnostic characters include: a digitate tail with a mucro and a glandular spermatheca filled with sperm in the first generation hermaphrodite; the value of D%; the morphology of the male spicules and gubernaculum and the number and arrangement of the genital papillae; the values of D%, E% and the pattern of the lateral field of the third-stage infective juvenile. Additionally, molecular evidence obtained from ITS rDNA RFLP profiles, 28S rDNA sequence analyses, and phylogenetic reconstruction provide further evidence to establish this nematode as a new species.
AB - Steinernema hermaphroditum n. sp., a new entomopathogenic nematode isolated from soil samples in the Moluccan islands, Indonesia, is described. Morphological observations as well as biological evidence (cross-hybridisation studies) indicate the distinctness of S. hermaphroditum n. sp. from other Steinernema spp. This new species is characterised by the presence of hermaphrodites in the first adult generation. Key morphological diagnostic characters include: a digitate tail with a mucro and a glandular spermatheca filled with sperm in the first generation hermaphrodite; the value of D%; the morphology of the male spicules and gubernaculum and the number and arrangement of the genital papillae; the values of D%, E% and the pattern of the lateral field of the third-stage infective juvenile. Additionally, molecular evidence obtained from ITS rDNA RFLP profiles, 28S rDNA sequence analyses, and phylogenetic reconstruction provide further evidence to establish this nematode as a new species.
KW - Description
KW - Hermaphroditism
KW - Molecular
KW - Phylogeny
KW - Taxonomy
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U2 - 10.1163/1568541042360555
DO - 10.1163/1568541042360555
M3 - Article
AN - SCOPUS:8444230962
VL - 6
SP - 401
EP - 412
JO - Nematology
JF - Nematology
SN - 1388-5545
IS - 3
ER -