Monoclonal antibody detection of CD46 clustering beneath Neisseria gonorrhoeae microcolonies

Nathan J. Weyand, Shaun W. Lee, Dustin L. Higashi, Daniel Cawley, Paul Yoshihara, Magdalene So

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


CD46 (membrane cofactor protein), a complement-regulatory protein that participates in innate and acquired immunity, also serves as a receptor for viral and bacterial pathogens. CD46 isoforms terminate in one of two cytoplasmic tails, Cyt1 or Cyt2, which differ in signaling and trafficking properties. Dissecting the functions of the two cytoplasmic tails in these cellular processes has been hampered by the absence of specific reagents. Here we report the construction of Cyt1- and Cyt2-specific monoclonal antibodies (MAbs). These MAbs recognize unique epitopes within the tails and can be used for immunofluorescence microscopy, immunoblotting, and immunoprecipitation. Studies of Neisseria gonorrhoeae-infected cells with the CD46 tail MAbs demonstrate the differential recruitment of Cyt1 and Cyt2 to the cortical plaque.

Original languageEnglish (US)
Pages (from-to)2428-2435
Number of pages8
JournalInfection and Immunity
Issue number4
StatePublished - Apr 2006

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases


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