Monoclonal antibodies to a penaeid shrimp parvovirus, infectious hypodermal and hematopoietic necrosis virus (ihhnv)

B. T. Poulos; D. V. Lightner; B. Trumper; J. R. Bonami

doi:10.1577/1548-8667(1994)006<0149:MATAPS>2.3.CO;2

Monoclonal antibodies to a penaeid shrimp parvovirus, infectious hypodermal and hematopoietic necrosis virus (ihhnv)

B. T. Poulos, D. V. Lightner, B. Trumper, J. R. Bonami

Research output: Contribution to journal › Article › peer-review

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Abstract

Murine monoclonal antibodies (MAbs) were developed to the parvovirus IHHNV, an important pathogen of cultured penaeid shrimp. An indirect enzyme-linked immunosorbent assay (ELISA) was standardized to evaluate six MAbs for their usefulness as rapid diagnostic tools for the identification of IHHNV in crude shrimp preparations and as tools for further study of the virus. Isotyping revealed that the MAbs were all immunoglobulin M (IgM) antibodies with kappa light chains. The MAbs distinguished between archived samples of IHHNV-infected and uninfected shrimp in the indirect ELISA. The six MAbs were also highly sensitive and specific when tested with purified IHHNV preparations by ELISA and immunoblot assays. Testing of fresh clinical specimens by ELISA indicated that some fresh crude shrimp extracts contain substances that bind murine antibodies nonspecifically, but these substances are not active in archived samples after long-term frozen storage.

Original language	English (US)
Pages (from-to)	149-154
Number of pages	6
Journal	Journal of Aquatic Animal Health
Volume	6
Issue number	2
DOIs	https://doi.org/10.1577/1548-8667(1994)006<0149:MATAPS>2.3.CO;2
State	Published - Jun 1994

ASJC Scopus subject areas

Aquatic Science

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10.1577/1548-8667(1994)006<0149:MATAPS>2.3.CO;2

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@article{a8e0f1c589ff4b08af694960ecc913f9,

title = "Monoclonal antibodies to a penaeid shrimp parvovirus, infectious hypodermal and hematopoietic necrosis virus (ihhnv)",

abstract = "Murine monoclonal antibodies (MAbs) were developed to the parvovirus IHHNV, an important pathogen of cultured penaeid shrimp. An indirect enzyme-linked immunosorbent assay (ELISA) was standardized to evaluate six MAbs for their usefulness as rapid diagnostic tools for the identification of IHHNV in crude shrimp preparations and as tools for further study of the virus. Isotyping revealed that the MAbs were all immunoglobulin M (IgM) antibodies with kappa light chains. The MAbs distinguished between archived samples of IHHNV-infected and uninfected shrimp in the indirect ELISA. The six MAbs were also highly sensitive and specific when tested with purified IHHNV preparations by ELISA and immunoblot assays. Testing of fresh clinical specimens by ELISA indicated that some fresh crude shrimp extracts contain substances that bind murine antibodies nonspecifically, but these substances are not active in archived samples after long-term frozen storage.",

author = "Poulos, {B. T.} and Lightner, {D. V.} and B. Trumper and Bonami, {J. R.}",

note = "Funding Information: We thank David Sammons, University of Arizona, Department of Veterinary Science, for his help in electrofusion, and Javier Enriquez and Deborah Bradley-Dunlop, University of Arizona Hybridoma Core Support Facility, for their assistance in developing monoclonal antibodies. Research was supported by grants from the U.S. De-partment of Agriculture's Gulf Coast Marine Shrimp Farming Consortium and from U.S. Department of Commerce, National Sea Grant Pro-gram.",

year = "1994",

month = jun,

doi = "10.1577/1548-8667(1994)006<0149:MATAPS>2.3.CO;2",

language = "English (US)",

volume = "6",

pages = "149--154",

journal = "Journal of Aquatic Animal Health",

issn = "0899-7659",

publisher = "John Wiley & Sons Inc.",

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AU - Poulos, B. T.

AU - Lightner, D. V.

AU - Trumper, B.

AU - Bonami, J. R.

N1 - Funding Information: We thank David Sammons, University of Arizona, Department of Veterinary Science, for his help in electrofusion, and Javier Enriquez and Deborah Bradley-Dunlop, University of Arizona Hybridoma Core Support Facility, for their assistance in developing monoclonal antibodies. Research was supported by grants from the U.S. De-partment of Agriculture's Gulf Coast Marine Shrimp Farming Consortium and from U.S. Department of Commerce, National Sea Grant Pro-gram.

PY - 1994/6

Y1 - 1994/6

N2 - Murine monoclonal antibodies (MAbs) were developed to the parvovirus IHHNV, an important pathogen of cultured penaeid shrimp. An indirect enzyme-linked immunosorbent assay (ELISA) was standardized to evaluate six MAbs for their usefulness as rapid diagnostic tools for the identification of IHHNV in crude shrimp preparations and as tools for further study of the virus. Isotyping revealed that the MAbs were all immunoglobulin M (IgM) antibodies with kappa light chains. The MAbs distinguished between archived samples of IHHNV-infected and uninfected shrimp in the indirect ELISA. The six MAbs were also highly sensitive and specific when tested with purified IHHNV preparations by ELISA and immunoblot assays. Testing of fresh clinical specimens by ELISA indicated that some fresh crude shrimp extracts contain substances that bind murine antibodies nonspecifically, but these substances are not active in archived samples after long-term frozen storage.

AB - Murine monoclonal antibodies (MAbs) were developed to the parvovirus IHHNV, an important pathogen of cultured penaeid shrimp. An indirect enzyme-linked immunosorbent assay (ELISA) was standardized to evaluate six MAbs for their usefulness as rapid diagnostic tools for the identification of IHHNV in crude shrimp preparations and as tools for further study of the virus. Isotyping revealed that the MAbs were all immunoglobulin M (IgM) antibodies with kappa light chains. The MAbs distinguished between archived samples of IHHNV-infected and uninfected shrimp in the indirect ELISA. The six MAbs were also highly sensitive and specific when tested with purified IHHNV preparations by ELISA and immunoblot assays. Testing of fresh clinical specimens by ELISA indicated that some fresh crude shrimp extracts contain substances that bind murine antibodies nonspecifically, but these substances are not active in archived samples after long-term frozen storage.

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Monoclonal antibodies to a penaeid shrimp parvovirus, infectious hypodermal and hematopoietic necrosis virus (ihhnv)

Abstract

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