Abstract
Rhodopsin has served as the primary model for studying G protein-coupled receptors (GPCRs)-the largest group in the human genome, and consequently a primary target for pharmaceutical development. Understanding the functions and activation mechanisms of GPCRs has proven to be extraordinarily difficult, as they are part of a complex signaling cascade and reside within the cell membrane. Although X-ray crystallography has recently solved several GPCR structures that may resemble the activated conformation, the dynamics and mechanism of rhodopsin activation continue to remain elusive. Notably solid-state 2H NMR spectroscopy provides key information pertinent to how local dynamics of the retinal ligand change during rhodopsin activation. When combined with molecular mechanics simulations of proteolipid membranes, a new paradigm for the rhodopsin activation process emerges. Experiment and simulation both suggest that retinal isomerization initiates the rhodopsin photocascade to yield not a single activated structure, but rather an ensemble of activated conformational states. This article is part of a Special Issue entitled: Membrane protein structure and function.
Original language | English (US) |
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Pages (from-to) | 241-251 |
Number of pages | 11 |
Journal | Biochimica et Biophysica Acta - Biomembranes |
Volume | 1818 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2012 |
Keywords
- G protein-coupled receptor
- Membrane
- Molecular dynamics
- Rhodopsin
- Solid-state NMR
- Vision
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Cell Biology