Molecular forms of immunoactive atrial natriuretic peptide in the rat hypothalamus and atrium

Christopher C. Glembotski, Gary M. Wildey, Thomas R. Gibson

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

Acid extracts of rat hypothalamus and atrium were prepared by a procedure previously shown to minimize proteolytic degradation of peptides. The majority of the immunoactive material in the atrial extracts had a molecular weight of approximately 9,000 to 15,000 daltons, while that in the hypothalamic extracts had a molecular weight of about 1,500 to 1,800 daltons. The major molecular weight forms of atrial natriuretic peptide from each extract were further distinguishable when analyzed by RP-HPLC. These results suggest that small peptides such as atriopeptins I, II, and III, may not be authentic post-translational processing products in the atrium, and that the hypothalamus and atrium may differentially cleave pro-atrial natriuretic peptide to form tissue-specific products.

Original languageEnglish (US)
Pages (from-to)671-678
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume129
Issue number3
DOIs
StatePublished - Jun 28 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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