Molecular cloning, sequencing, chromosomal localization, and tissue distribution of the human Na+/H+exchanger (SLC9A2)

Fayez K. Ghishan; Susan M. Knobel; Marshall Summar

doi:10.1006/geno.1995.0004

Molecular cloning, sequencing, chromosomal localization, and tissue distribution of the human Na⁺/H⁺exchanger (SLC9A2)

Fayez K. Ghishan, Susan M. Knobel, Marshall Summar

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The Na⁺/H⁺exchangers belong to a family of transport proteins involved in intracellular pH regulation and vectorial sodium transport across various epithelial tissues. We have cloned a unique isoform (NHE-2) by screening a rat intestinal cDNA library utilizing a human NHE-1 fragment. In this report we describe molecular cloning of the human Na⁺/H⁺exchanger (NHE-2, HGMW-approved symbol SLC9A2). The cDNA encodes a protein of 698 amino acid residues. Human NHE-2 is widely distributed in tissues of the gastrointestinal tract, kidney, heart, testes, uterus, and adrenal glands. Hydropathy plot indicates that the translated protein is predicted to have 10 transmembrane domains with three potential N-glycosylation sites and four potential cAMP- and cGMP-dependent protein kinase phosphorylation sites. This cDNA maps the location of the gene encoding NHE-2 to human chromosome 2q11.2.

Original language	English (US)
Pages (from-to)	25-30
Number of pages	6
Journal	Genomics
Volume	30
Issue number	1
DOIs	https://doi.org/10.1006/geno.1995.0004
State	Published - Nov 1995
Externally published	Yes

ASJC Scopus subject areas

Genetics

Access to Document

10.1006/geno.1995.0004

Cite this

@article{56db3f0af1424dc4890daa95a0e1ddc7,

title = "Molecular cloning, sequencing, chromosomal localization, and tissue distribution of the human Na+/H+exchanger (SLC9A2)",

abstract = "The Na+/H+exchangers belong to a family of transport proteins involved in intracellular pH regulation and vectorial sodium transport across various epithelial tissues. We have cloned a unique isoform (NHE-2) by screening a rat intestinal cDNA library utilizing a human NHE-1 fragment. In this report we describe molecular cloning of the human Na+/H+exchanger (NHE-2, HGMW-approved symbol SLC9A2). The cDNA encodes a protein of 698 amino acid residues. Human NHE-2 is widely distributed in tissues of the gastrointestinal tract, kidney, heart, testes, uterus, and adrenal glands. Hydropathy plot indicates that the translated protein is predicted to have 10 transmembrane domains with three potential N-glycosylation sites and four potential cAMP- and cGMP-dependent protein kinase phosphorylation sites. This cDNA maps the location of the gene encoding NHE-2 to human chromosome 2q11.2.",

author = "Ghishan, {Fayez K.} and Knobel, {Susan M.} and Marshall Summar",

note = "Funding Information: This work was supported by NIH Grant 2R01 DK 41274 and the Arizona Elks members and families.",

year = "1995",

month = nov,

doi = "10.1006/geno.1995.0004",

language = "English (US)",

volume = "30",

pages = "25--30",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Molecular cloning, sequencing, chromosomal localization, and tissue distribution of the human Na+/H+exchanger (SLC9A2)

AU - Ghishan, Fayez K.

AU - Knobel, Susan M.

AU - Summar, Marshall

N1 - Funding Information: This work was supported by NIH Grant 2R01 DK 41274 and the Arizona Elks members and families.

PY - 1995/11

Y1 - 1995/11

N2 - The Na+/H+exchangers belong to a family of transport proteins involved in intracellular pH regulation and vectorial sodium transport across various epithelial tissues. We have cloned a unique isoform (NHE-2) by screening a rat intestinal cDNA library utilizing a human NHE-1 fragment. In this report we describe molecular cloning of the human Na+/H+exchanger (NHE-2, HGMW-approved symbol SLC9A2). The cDNA encodes a protein of 698 amino acid residues. Human NHE-2 is widely distributed in tissues of the gastrointestinal tract, kidney, heart, testes, uterus, and adrenal glands. Hydropathy plot indicates that the translated protein is predicted to have 10 transmembrane domains with three potential N-glycosylation sites and four potential cAMP- and cGMP-dependent protein kinase phosphorylation sites. This cDNA maps the location of the gene encoding NHE-2 to human chromosome 2q11.2.

AB - The Na+/H+exchangers belong to a family of transport proteins involved in intracellular pH regulation and vectorial sodium transport across various epithelial tissues. We have cloned a unique isoform (NHE-2) by screening a rat intestinal cDNA library utilizing a human NHE-1 fragment. In this report we describe molecular cloning of the human Na+/H+exchanger (NHE-2, HGMW-approved symbol SLC9A2). The cDNA encodes a protein of 698 amino acid residues. Human NHE-2 is widely distributed in tissues of the gastrointestinal tract, kidney, heart, testes, uterus, and adrenal glands. Hydropathy plot indicates that the translated protein is predicted to have 10 transmembrane domains with three potential N-glycosylation sites and four potential cAMP- and cGMP-dependent protein kinase phosphorylation sites. This cDNA maps the location of the gene encoding NHE-2 to human chromosome 2q11.2.

UR - http://www.scopus.com/inward/record.url?scp=0028804108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028804108&partnerID=8YFLogxK

U2 - 10.1006/geno.1995.0004

DO - 10.1006/geno.1995.0004

M3 - Article

C2 - 8595899

AN - SCOPUS:0028804108

SN - 0888-7543

VL - 30

SP - 25

EP - 30

JO - Genomics

JF - Genomics

IS - 1

ER -

Molecular cloning, sequencing, chromosomal localization, and tissue distribution of the human Na⁺/H⁺exchanger (SLC9A2)

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this