Molecular cloning, sequencing, and expression of equine interleukin-6

C. E. Swiderski, G. Sobol, D. P. Lunn, D. W. Horohov

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Equine interleukin-6 (IL-6) cDNA was amplified from mitogen-stimulated equine peripheral blood mononuclear cells (PBMC) using consensus sequence primers. The 727 bp amplified cDNA contains the entire coding region for equine IL-6 and includes 118 bases in the 3′ non-translated region. The coding sequence translates to a protein of 208 amino acids with a predicted 28 amino acid leader sequence. The mature protein of 180 amino acids has a predicted molecular mass of 20 471 Da without post-translational modifications. The amino acid sequence of equine IL-6 displays between 46 and 84% similarity to other mammalian IL-6 sequences. Expression of equine IL-6 in Chinese hamster ovary (CHO) cells yielded a supernatant that supported the proliferation of B9 cells in a dose-dependent manner. Treatment of B9 cells with an anti-IL-6 receptor antibody ablated the response to the recombinant equine IL-6.

Original languageEnglish (US)
Pages (from-to)213-220
Number of pages8
JournalVeterinary Immunology and Immunopathology
Volume77
Issue number3-4
DOIs
StatePublished - Dec 29 2000
Externally publishedYes

Keywords

  • Equine interleukin-6 (IL-6) cloning expression

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

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