Molecular cloning of murine sodium-phosphate cotransporter type IIb (Na/P(i)-IIb) gene promoter and characterization of gene structure

Kayo Arima; James F. Collins; Eric R. Hines; Liqun Bai; Fayez K. Ghishan

doi:10.1016/S0167-4781(00)00205-0

Molecular cloning of murine sodium-phosphate cotransporter type IIb (Na/P(i)-IIb) gene promoter and characterization of gene structure

Kayo Arima, James F. Collins, Eric R. Hines, Liqun Bai, Fayez K. Ghishan

Research output: Contribution to journal › Article › peer-review

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Abstract

We report the cloning of the murine Na/P(i)-IIb cotransporter gene, which spans more than 18 kilobases and consists of 12 introns and 13 exons. Three promoter/reporter gene constructs, -159/+73, -429/+73 and -954/+73, showed significant luciferase activity (22-82-fold over background) when transfected into in rat intestinal epithelial (RIE-1) cells. Copyright (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	149-154
Number of pages	6
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1494
Issue number	1-2
DOIs	https://doi.org/10.1016/S0167-4781(00)00205-0
State	Published - Nov 15 2000

Keywords

Intestine
Promoter analysis
Sodium-phosphate transporter

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(00)00205-0

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Molecular cloning of murine sodium-phosphate cotransporter type IIb (Na/P(i)-IIb) gene promoter and characterization of gene structure. / Arima, Kayo; Collins, James F.; Hines, Eric R. et al.
In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1494, No. 1-2, 15.11.2000, p. 149-154.

Research output: Contribution to journal › Article › peer-review

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abstract = "We report the cloning of the murine Na/P(i)-IIb cotransporter gene, which spans more than 18 kilobases and consists of 12 introns and 13 exons. Three promoter/reporter gene constructs, -159/+73, -429/+73 and -954/+73, showed significant luciferase activity (22-82-fold over background) when transfected into in rat intestinal epithelial (RIE-1) cells. Copyright (C) 2000 Elsevier Science B.V.",

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AU - Bai, Liqun

AU - Ghishan, Fayez K.

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KW - Promoter analysis

KW - Sodium-phosphate transporter

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Molecular cloning of murine sodium-phosphate cotransporter type IIb (Na/P(i)-IIb) gene promoter and characterization of gene structure

Abstract

Keywords

ASJC Scopus subject areas

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