Molecular cloning of a unique human T-cell leukemia virus (HTLV-II(Mo))

E. P. Gelmann, G. Franchini, V. Manzari, F. Wong-Staal, R. C. Gallo

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Human T-cell leukemia virus II(Mo) (HTLV-II(Mo)) is a human retrovirus isolated from a patient with a T-cell hairy cell leukemia. This virus has been shown to have core protein (gag) antigens similar to, but distinct from, those of all known isolates of the prototype human T-cell leukemia virus (HTLV-I). We have used a subgenomic clone of the HTLV-I env-pX region to detect and characterize HTLV-II(Mo) proviral sequences by performing Southern blot hybridization under conditions of low stringency. Using the HTLV-I probe, we cloned a partial integrated HTLV-II(Mo) provirus from a genomic library of the producer Mo cell line. These sequences could be characterized by low stringency hybridization with different subgenomic clones of HTLV-I. An HTLV-II(Mo)-specific subclone was made by isolating a 3.6-kilobase BamHI fragment of the partial provirus. This was used to clone two full-length integrated viral genomes. Using the HTLV-II(Mo) viral probe, we also showed by hybridization under stringent conditions to DNA and RNA of various infected and uninfected cell lines that these HTLV-II(Mo) sequences are unique.

Original languageEnglish (US)
Pages (from-to)993-997
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number4 I
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

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