Molecular cloning of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter

L. Bai; J. F. Collins; H. Xu; L. Xu; F. K. Ghishan

doi:10.1016/S0167-4781(01)00297-4

Molecular cloning of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter

L. Bai
, J. F. Collins
, H. Xu
, L. Xu
, F. K. Ghishan

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We report the novel cloning and preliminary characterization of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter. Five promoter/luciferase reporter gene constructs, -1816/+61, -1620/+61, -1223/+61, -600/+61 and -225/+61, showed significant luciferase activity (6-14-fold over background) when transfected into human colon carcinoma (Caco-2) and opossum kidney (OKP) cells.

Original language	English (US)
Pages (from-to)	42-45
Number of pages	4
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1522
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4781(01)00297-4
State	Published - Nov 11 2001

Keywords

Caco-2 cell
Gene regulation
Intestine
OKP cell
Ram-1
Sodium-phosphate cotransporter

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(01)00297-4

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abstract = "We report the novel cloning and preliminary characterization of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter. Five promoter/luciferase reporter gene constructs, -1816/+61, -1620/+61, -1223/+61, -600/+61 and -225/+61, showed significant luciferase activity (6-14-fold over background) when transfected into human colon carcinoma (Caco-2) and opossum kidney (OKP) cells.",

keywords = "Caco-2 cell, Gene regulation, Intestine, OKP cell, Ram-1, Sodium-phosphate cotransporter",

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AU - Bai, L.

AU - Collins, J. F.

AU - Xu, H.

AU - Xu, L.

AU - Ghishan, F. K.

PY - 2001/11/11

Y1 - 2001/11/11

N2 - We report the novel cloning and preliminary characterization of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter. Five promoter/luciferase reporter gene constructs, -1816/+61, -1620/+61, -1223/+61, -600/+61 and -225/+61, showed significant luciferase activity (6-14-fold over background) when transfected into human colon carcinoma (Caco-2) and opossum kidney (OKP) cells.

AB - We report the novel cloning and preliminary characterization of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter. Five promoter/luciferase reporter gene constructs, -1816/+61, -1620/+61, -1223/+61, -600/+61 and -225/+61, showed significant luciferase activity (6-14-fold over background) when transfected into human colon carcinoma (Caco-2) and opossum kidney (OKP) cells.

KW - Caco-2 cell

KW - Gene regulation

KW - Intestine

KW - OKP cell

KW - Ram-1

KW - Sodium-phosphate cotransporter

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AN - SCOPUS:0035846444

SN - 0167-4781

VL - 1522

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EP - 45

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 1

ER -

Molecular cloning of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter

Abstract

Keywords

ASJC Scopus subject areas

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