Abstract
We report the novel cloning and preliminary characterization of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter. Five promoter/luciferase reporter gene constructs, -1816/+61, -1620/+61, -1223/+61, -600/+61 and -225/+61, showed significant luciferase activity (6-14-fold over background) when transfected into human colon carcinoma (Caco-2) and opossum kidney (OKP) cells.
Original language | English (US) |
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Pages (from-to) | 42-45 |
Number of pages | 4 |
Journal | Biochimica et Biophysica Acta - Gene Structure and Expression |
Volume | 1522 |
Issue number | 1 |
DOIs | |
State | Published - Nov 11 2001 |
Keywords
- Caco-2 cell
- Gene regulation
- Intestine
- OKP cell
- Ram-1
- Sodium-phosphate cotransporter
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics