TY - JOUR
T1 - Molecular cloning and expression analysis of soluble and membrane-bound trehalase genes in the cotton bollworm, Helicoverpa armigera
AU - Ma, Long
AU - Dai, Wu
AU - Li, Xianchun
AU - Zhang, Yalin
AU - Zhang, Chunni
N1 - Funding Information:
We thank Christopher H. Dietrich (Illinois Natural History Survey, University of Illinois at Urbana-Champaign, IL, USA) and John Richard Schrock (Emporia State University, Emporia, KS, USA) for comments on an earlier draft of this paper. This work was supported by the National Natural Science Foundation of China ( 31101441 ) and the “ Chinese Universities Scientific Fund ( QN2013012 )”.
Publisher Copyright:
© 2015 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - Trehalase plays a significant role in various physiological processes in insects. In this study, we cloned and characterized a soluble trehalase gene (HaTreh-1) and a membrane-bound trehalase gene (HaTreh-2) from Helicoverpa armigera, a serious polyphagous pest of crops. HaTreh-1 contained an open reading frame of 1716. bp that encodes a protein of 572 amino acids. HaTreh-2 has an open reading frame of 1938. bp, encoding a protein of 646 amino acids. Sequence alignment and phylogenetic analysis of the two putative proteins revealed that HaTreh-1 and HaTreh-2 belong to soluble and membrane-bound trehalase groups, respectively. Quantitative real-time PCR (qPCR) analyses of the spatiotemporal expression pattern of HaTreh in H. armigera revealed that HaTreh-1 was expressed mainly in the midgut, with lower expression in the integument and head, Malpighian tubules, trachea, and fat body. The expression levels of HaTreh-2 were detected in all 6 tissues, and HaTreh-2 was mainly expressed in the midgut and head. Expression of HaTreh-1 was higher throughout the larval stages, but lower on days 1 and 2 of the pupal stage. Expression of HaTreh-2 was higher during the 4th- and 5th-instar larval stages. Taken together, these results suggest that HaTreh-1 and HaTreh-2 have different functions in various developmental stages and tissues.
AB - Trehalase plays a significant role in various physiological processes in insects. In this study, we cloned and characterized a soluble trehalase gene (HaTreh-1) and a membrane-bound trehalase gene (HaTreh-2) from Helicoverpa armigera, a serious polyphagous pest of crops. HaTreh-1 contained an open reading frame of 1716. bp that encodes a protein of 572 amino acids. HaTreh-2 has an open reading frame of 1938. bp, encoding a protein of 646 amino acids. Sequence alignment and phylogenetic analysis of the two putative proteins revealed that HaTreh-1 and HaTreh-2 belong to soluble and membrane-bound trehalase groups, respectively. Quantitative real-time PCR (qPCR) analyses of the spatiotemporal expression pattern of HaTreh in H. armigera revealed that HaTreh-1 was expressed mainly in the midgut, with lower expression in the integument and head, Malpighian tubules, trachea, and fat body. The expression levels of HaTreh-2 were detected in all 6 tissues, and HaTreh-2 was mainly expressed in the midgut and head. Expression of HaTreh-1 was higher throughout the larval stages, but lower on days 1 and 2 of the pupal stage. Expression of HaTreh-2 was higher during the 4th- and 5th-instar larval stages. Taken together, these results suggest that HaTreh-1 and HaTreh-2 have different functions in various developmental stages and tissues.
KW - Cloning
KW - Expression pattern
KW - Helicoverpa armigera
KW - Membrane-bound trehalase
KW - Quantitative real-time PCR
KW - Soluble trehalase
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U2 - 10.1016/j.aspen.2015.01.002
DO - 10.1016/j.aspen.2015.01.002
M3 - Article
AN - SCOPUS:84922270486
SN - 1226-8615
VL - 18
SP - 187
EP - 195
JO - Journal of Asia-Pacific Entomology
JF - Journal of Asia-Pacific Entomology
IS - 2
ER -