Molecular cloning and characterization of a human urate transporter (hURAT1) gene promoter

Tao Li; Jacob R. Walsh; Fayez K. Ghishan; Liqun Bai

doi:10.1016/j.bbaexp.2004.10.001

Molecular cloning and characterization of a human urate transporter (hURAT1) gene promoter

Tao Li, Jacob R. Walsh, Fayez K. Ghishan, Liqun Bai

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

We report the novel cloning and preliminary characterization of a human urate transporter (hURAT1) gene promoter. The transcription initiation site was mapped to a base 337 bp upstream of the ATG start codon by primer extension and 5′-RACE. The minimal functional promoter region is within 253 bp when the promoter/luciferase constructs were transfected into OK cells. The sex hormone testosterone significantly increases promoter activity, suggesting that hormonal regulation of hURAT1 is the root cause of observed differences in urate levels between males and females.

Original language	English (US)
Pages (from-to)	53-58
Number of pages	6
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1681
Issue number	1
DOIs	https://doi.org/10.1016/j.bbaexp.2004.10.001
State	Published - Nov 24 2004

Keywords

Organic anion transporter
Promoter
Testosterone
URAT1
Uric acid transport

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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abstract = "We report the novel cloning and preliminary characterization of a human urate transporter (hURAT1) gene promoter. The transcription initiation site was mapped to a base 337 bp upstream of the ATG start codon by primer extension and 5′-RACE. The minimal functional promoter region is within 253 bp when the promoter/luciferase constructs were transfected into OK cells. The sex hormone testosterone significantly increases promoter activity, suggesting that hormonal regulation of hURAT1 is the root cause of observed differences in urate levels between males and females.",

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AU - Walsh, Jacob R.

AU - Ghishan, Fayez K.

AU - Bai, Liqun

PY - 2004/11/24

Y1 - 2004/11/24

N2 - We report the novel cloning and preliminary characterization of a human urate transporter (hURAT1) gene promoter. The transcription initiation site was mapped to a base 337 bp upstream of the ATG start codon by primer extension and 5′-RACE. The minimal functional promoter region is within 253 bp when the promoter/luciferase constructs were transfected into OK cells. The sex hormone testosterone significantly increases promoter activity, suggesting that hormonal regulation of hURAT1 is the root cause of observed differences in urate levels between males and females.

AB - We report the novel cloning and preliminary characterization of a human urate transporter (hURAT1) gene promoter. The transcription initiation site was mapped to a base 337 bp upstream of the ATG start codon by primer extension and 5′-RACE. The minimal functional promoter region is within 253 bp when the promoter/luciferase constructs were transfected into OK cells. The sex hormone testosterone significantly increases promoter activity, suggesting that hormonal regulation of hURAT1 is the root cause of observed differences in urate levels between males and females.

KW - Organic anion transporter

KW - Promoter

KW - Testosterone

KW - URAT1

KW - Uric acid transport

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

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Molecular cloning and characterization of a human urate transporter (hURAT1) gene promoter

Abstract

Keywords

ASJC Scopus subject areas

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