Modeling column regeneration effects on ion-exchange chromatography

Z. Karina López; Armando Tejeda; Rosa M. Montesinos; Ignacio Magaña; Roberto Guzmán

doi:10.1016/S0021-9673(97)00841-8

Modeling column regeneration effects on ion-exchange chromatography

Z. Karina López, Armando Tejeda, Rosa M. Montesinos, Ignacio Magaña, Roberto Guzmán

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The effect of in-place regeneration on equilibrium and kinetic characteristics of the adsorption of bovine serum albumin to a DEAE-cellulose anion exchanger has been determined. Regeneration with sodium hydroxide and time of exposure showed no effect on equilibrium behavior. Breakthrough curves were measured for protein adsorption on fixed-bed columns and analyzed by a simple model to determine the relevant rate constants for the adsorption process. It was found that forward adsorption rate constant decreased exponentially with the chemical treatment exposure time. The implications of the results on the design and optimization of ion-exchange chromatographic processes are discussed.

Original language	English (US)
Pages (from-to)	99-107
Number of pages	9
Journal	Journal of Chromatography A
Volume	791
Issue number	1-2
DOIs	https://doi.org/10.1016/S0021-9673(97)00841-8
State	Published - Dec 12 1997

Keywords

Adsorption isotherms
Albumin
Column regeneration
Computer simulation
Ion-exchange chromatography
Kinetic studies
Proteins

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(97)00841-8

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title = "Modeling column regeneration effects on ion-exchange chromatography",

abstract = "The effect of in-place regeneration on equilibrium and kinetic characteristics of the adsorption of bovine serum albumin to a DEAE-cellulose anion exchanger has been determined. Regeneration with sodium hydroxide and time of exposure showed no effect on equilibrium behavior. Breakthrough curves were measured for protein adsorption on fixed-bed columns and analyzed by a simple model to determine the relevant rate constants for the adsorption process. It was found that forward adsorption rate constant decreased exponentially with the chemical treatment exposure time. The implications of the results on the design and optimization of ion-exchange chromatographic processes are discussed.",

keywords = "Adsorption isotherms, Albumin, Column regeneration, Computer simulation, Ion-exchange chromatography, Kinetic studies, Proteins",

author = "L{\'o}pez, {Z. Karina} and Armando Tejeda and Montesinos, {Rosa M.} and Ignacio Maga{\~n}a and Roberto Guzm{\'a}n",

note = "Funding Information: The work described here was supported by a grant from the Consejo Nacional de Ciencia y Tecnolog{\'ı}a, Mexico (CONACyT: 2046-A9302).",

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doi = "10.1016/S0021-9673(97)00841-8",

language = "English (US)",

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T1 - Modeling column regeneration effects on ion-exchange chromatography

AU - López, Z. Karina

AU - Tejeda, Armando

AU - Montesinos, Rosa M.

AU - Magaña, Ignacio

AU - Guzmán, Roberto

N1 - Funding Information: The work described here was supported by a grant from the Consejo Nacional de Ciencia y Tecnologı́a, Mexico (CONACyT: 2046-A9302).

PY - 1997/12/12

Y1 - 1997/12/12

N2 - The effect of in-place regeneration on equilibrium and kinetic characteristics of the adsorption of bovine serum albumin to a DEAE-cellulose anion exchanger has been determined. Regeneration with sodium hydroxide and time of exposure showed no effect on equilibrium behavior. Breakthrough curves were measured for protein adsorption on fixed-bed columns and analyzed by a simple model to determine the relevant rate constants for the adsorption process. It was found that forward adsorption rate constant decreased exponentially with the chemical treatment exposure time. The implications of the results on the design and optimization of ion-exchange chromatographic processes are discussed.

AB - The effect of in-place regeneration on equilibrium and kinetic characteristics of the adsorption of bovine serum albumin to a DEAE-cellulose anion exchanger has been determined. Regeneration with sodium hydroxide and time of exposure showed no effect on equilibrium behavior. Breakthrough curves were measured for protein adsorption on fixed-bed columns and analyzed by a simple model to determine the relevant rate constants for the adsorption process. It was found that forward adsorption rate constant decreased exponentially with the chemical treatment exposure time. The implications of the results on the design and optimization of ion-exchange chromatographic processes are discussed.

KW - Adsorption isotherms

KW - Albumin

KW - Column regeneration

KW - Computer simulation

KW - Ion-exchange chromatography

KW - Kinetic studies

KW - Proteins

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U2 - 10.1016/S0021-9673(97)00841-8

DO - 10.1016/S0021-9673(97)00841-8

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AN - SCOPUS:0345267066

SN - 0021-9673

VL - 791

SP - 99

EP - 107

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 1-2

ER -

Modeling column regeneration effects on ion-exchange chromatography

Abstract

Keywords

ASJC Scopus subject areas

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