@article{5a38089b10344e0287f0eb6f7111dd4d,
title = "miR-106b suppresses pathological retinal angiogenesis",
abstract = "MicroRNAs are small non-coding RNAs that post-transcriptionally regulate gene expression. We recently demonstrated that levels of miR-106b were significantly decreased in the vitreous and plasma of patients with neovascular age-related macular degeneration (AMD). Here we show that expression of the miR-106b-25 cluster is negatively regulated by the unfolded protein response pathway of protein kinase RNA-like ER kinase (PERK) in a mouse model of neovascular AMD. A reduction in levels of miR-106b triggers vascular growth both in vivo and in vitro by inducing production of pro-angiogenic factors. We demonstrate that therapeutic delivery of miR-106b to the retina with lentiviral vectors protects against aberrant retinal angiogenesis in two distinct mouse models of pathological retinal neovascularization. Results from this study suggest that miRNAs such as miR-106b have the potential to be used as multitarget therapeutics for conditions characterized by pathological retinal angiogenesis.",
keywords = "PERK, age related macular degeneration, angiogenesis, choroidal neovascularization, miR-106b",
author = "Cath{\'e}rine M{\'e}nard and Wilson, {Ariel M.} and Agnieszka Dejda and Khalil Miloudi and Fran{\c c}ois Binet and Sergio Crespo-Garcia and C{\'e}lia Parinot and Fr{\'e}d{\'e}rique Pilon and Rachel Juneau and Andriessen, {Elisabeth MMA} and Ga{\"e}lle Mawambo and SanGiovanni, {John Paul} and Guire, {Vincent De} and Przemyslaw Sapieha",
note = "Funding Information: This work was supported by operating grants from The Foundation Fighting Blindness Canada, the Canadian Diabetes Association (DI-3-18-5444-PS), the Canadian Institutes of Health Research (Foundation grant #353770 to P.S), the Canadian the Heart and Stroke Foundation Canada (G-16-00014658 to P.S.), and Natural Sciences and Engineering Research Council of Canada (418637 to P.S.). P.S. holds the Wolfe Professorship in Translational Research and a Canada Research Chair in Retinal Cell Biology. We also thank the Fonds de Recherche en Ophtalmologie de l{\textquoteright}Universit{\'e} de Montr{\'e}al (FROUM) and the RRSV. Funding Information: This work was supported by operating grants from The Foundation Fighting Blindness Canada, the Canadian Diabetes Association (DI-3-18-5444-PS), the Canadian Institutes of Health Research (Foundation grant #353770 to P.S), the Canadian the Heart and Stroke Foundation Canada (G-16-00014658 to P.S.), and Natural Sciences and Engineering Research Council of Canada (418637 to P.S.). P.S. holds the Wolfe Professorship in Translational Research and a Canada Research Chair in Retinal Cell Biology. We also thank the Fonds de Recherche en Ophtalmologie de l{\textquoteright}Universite de Montreal (FROUM) and the RRSV. Publisher Copyright: {\textcopyright} 2020 M{\'e}nard et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2020",
month = dec,
day = "31",
doi = "10.18632/aging.202404",
language = "English (US)",
volume = "12",
pages = "24836--24852",
journal = "Aging",
issn = "1945-4589",
publisher = "Impact Journals LLC",
number = "24",
}