Abstract
An endoscopic system that provides simultaneous cross-sectional imaging and fluorescence spectroscopy is described. The first application of this device was the investigation of mouse colon cancer in vivo. This system combined optical coherence tomography (OCT), which provided high-resolution cross-sectional structural information in the form of a two-dimensional image, and laser induced fluorescence (LIF), which yielded histochemical information about the tissue. The design challenge and solution of packaging these two systems with widely different optical requirements are described in detail. The illumination geometry of the endoscope was similar to earlier published OCT and LIF catheter endoscope designs. However, several unique design challenges encountered in combining these two systems have been addressed. The use of a rodprism to reduce the asymmetry in the OCT beam caused by a cylindrical window is presented. Materials selection for use with wavelengths from 325nm - 1310nm presented a challenge usually avoided in OCT endoscopes. Preliminary mouse colon data collected with this endoscopic device is compared with previous experiments performed by researchers in our lab working with an earlier bulk-optic, combined OCT-LIF system.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 129-138 |
| Number of pages | 10 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 4956 |
| DOIs | |
| State | Published - 2003 |
| Event | PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII - San Jose, CA, United States Duration: Jan 27 2003 → Jan 29 2003 |
Keywords
- Catheter
- Colon
- Laser induced fluorescence
- Mouse
- Multi-modal
- Optical coherence tomography
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering