Mimicry of Canonical Translation Elongation Underlies Alanine Tail Synthesis in RQC

Sebastian Filbeck; Federico Cerullo; Helge Paternoga; George Tsaprailis; Claudio A.P. Joazeiro; Stefan Pfeffer

doi:10.1016/j.molcel.2020.11.001

Mimicry of Canonical Translation Elongation Underlies Alanine Tail Synthesis in RQC

Sebastian Filbeck
, Federico Cerullo
, Helge Paternoga
, George Tsaprailis
, Claudio A.P. Joazeiro
, Stefan Pfeffer

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Incompletely made nascent chains are tagged for proteolysis by ribosome-associated quality control (RQC). In bacterial RQC, this is mediated by C-terminal alanine tailing. Filbeck et al. elucidate the structural basis for alanine tailing using cryo-EM, discover Hsp15/RqcP as an essential factor, and reveal that C-terminal tailing follows similar principles to canonical translation elongation.

Original language	English (US)
Pages (from-to)	104-114.e6
Journal	Molecular cell
Volume	81
Issue number	1
DOIs	https://doi.org/10.1016/j.molcel.2020.11.001
State	Published - Jan 7 2021

Keywords

Hsp15
RQC
RqcH
RqcP
SsrA
alanine tailing
cryo-EM
ribosome-associated quality control
ribosomes
translation elongation

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/j.molcel.2020.11.001

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title = "Mimicry of Canonical Translation Elongation Underlies Alanine Tail Synthesis in RQC",

abstract = "Incompletely made nascent chains are tagged for proteolysis by ribosome-associated quality control (RQC). In bacterial RQC, this is mediated by C-terminal alanine tailing. Filbeck et al. elucidate the structural basis for alanine tailing using cryo-EM, discover Hsp15/RqcP as an essential factor, and reveal that C-terminal tailing follows similar principles to canonical translation elongation.",

keywords = "Hsp15, RQC, RqcH, RqcP, SsrA, alanine tailing, cryo-EM, ribosome-associated quality control, ribosomes, translation elongation",

author = "Sebastian Filbeck and Federico Cerullo and Helge Paternoga and George Tsaprailis and Joazeiro, \{Claudio A.P.\} and Stefan Pfeffer",

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AU - Filbeck, Sebastian

AU - Cerullo, Federico

AU - Paternoga, Helge

AU - Tsaprailis, George

AU - Joazeiro, Claudio A.P.

AU - Pfeffer, Stefan

PY - 2021/1/7

Y1 - 2021/1/7

N2 - Incompletely made nascent chains are tagged for proteolysis by ribosome-associated quality control (RQC). In bacterial RQC, this is mediated by C-terminal alanine tailing. Filbeck et al. elucidate the structural basis for alanine tailing using cryo-EM, discover Hsp15/RqcP as an essential factor, and reveal that C-terminal tailing follows similar principles to canonical translation elongation.

AB - Incompletely made nascent chains are tagged for proteolysis by ribosome-associated quality control (RQC). In bacterial RQC, this is mediated by C-terminal alanine tailing. Filbeck et al. elucidate the structural basis for alanine tailing using cryo-EM, discover Hsp15/RqcP as an essential factor, and reveal that C-terminal tailing follows similar principles to canonical translation elongation.

KW - Hsp15

KW - RQC

KW - RqcH

KW - RqcP

KW - SsrA

KW - alanine tailing

KW - cryo-EM

KW - ribosome-associated quality control

KW - ribosomes

KW - translation elongation

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UR - https://www.scopus.com/pages/publications/85097872385#tab=citedBy

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M3 - Article

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AN - SCOPUS:85097872385

SN - 1097-2765

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SP - 104-114.e6

JO - Molecular cell

JF - Molecular cell

IS - 1

ER -

Mimicry of Canonical Translation Elongation Underlies Alanine Tail Synthesis in RQC

Abstract

Keywords

ASJC Scopus subject areas

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