Mimicry of Canonical Translation Elongation Underlies Alanine Tail Synthesis in RQC

Sebastian Filbeck; Federico Cerullo; Helge Paternoga; George Tsaprailis; Claudio A.P. Joazeiro; Stefan Pfeffer

doi:10.1016/j.molcel.2020.11.001

Mimicry of Canonical Translation Elongation Underlies Alanine Tail Synthesis in RQC

Sebastian Filbeck, Federico Cerullo, Helge Paternoga, George Tsaprailis, Claudio A.P. Joazeiro, Stefan Pfeffer

Toxicology, Center for

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Incompletely made nascent chains are tagged for proteolysis by ribosome-associated quality control (RQC). In bacterial RQC, this is mediated by C-terminal alanine tailing. Filbeck et al. elucidate the structural basis for alanine tailing using cryo-EM, discover Hsp15/RqcP as an essential factor, and reveal that C-terminal tailing follows similar principles to canonical translation elongation.

Original language	English (US)
Pages (from-to)	104-114.e6
Journal	Molecular cell
Volume	81
Issue number	1
DOIs	https://doi.org/10.1016/j.molcel.2020.11.001
State	Published - Jan 7 2021

Keywords

Hsp15
RQC
RqcH
RqcP
SsrA
alanine tailing
cryo-EM
ribosome-associated quality control
ribosomes
translation elongation

ASJC Scopus subject areas

Molecular Biology
Cell Biology

Access to Document

10.1016/j.molcel.2020.11.001

Cite this

@article{3794ab165de14afe9616cf20cc1d223a,

title = "Mimicry of Canonical Translation Elongation Underlies Alanine Tail Synthesis in RQC",

abstract = "Incompletely made nascent chains are tagged for proteolysis by ribosome-associated quality control (RQC). In bacterial RQC, this is mediated by C-terminal alanine tailing. Filbeck et al. elucidate the structural basis for alanine tailing using cryo-EM, discover Hsp15/RqcP as an essential factor, and reveal that C-terminal tailing follows similar principles to canonical translation elongation.",

keywords = "Hsp15, RQC, RqcH, RqcP, SsrA, alanine tailing, cryo-EM, ribosome-associated quality control, ribosomes, translation elongation",

author = "Sebastian Filbeck and Federico Cerullo and Helge Paternoga and George Tsaprailis and Joazeiro, {Claudio A.P.} and Stefan Pfeffer",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2021",

month = jan,

day = "7",

doi = "10.1016/j.molcel.2020.11.001",

language = "English (US)",

volume = "81",

pages = "104--114.e6",

journal = "Molecular cell",

issn = "1097-2765",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - Mimicry of Canonical Translation Elongation Underlies Alanine Tail Synthesis in RQC

AU - Filbeck, Sebastian

AU - Cerullo, Federico

AU - Paternoga, Helge

AU - Tsaprailis, George

AU - Joazeiro, Claudio A.P.

AU - Pfeffer, Stefan

PY - 2021/1/7

Y1 - 2021/1/7

N2 - Incompletely made nascent chains are tagged for proteolysis by ribosome-associated quality control (RQC). In bacterial RQC, this is mediated by C-terminal alanine tailing. Filbeck et al. elucidate the structural basis for alanine tailing using cryo-EM, discover Hsp15/RqcP as an essential factor, and reveal that C-terminal tailing follows similar principles to canonical translation elongation.

AB - Incompletely made nascent chains are tagged for proteolysis by ribosome-associated quality control (RQC). In bacterial RQC, this is mediated by C-terminal alanine tailing. Filbeck et al. elucidate the structural basis for alanine tailing using cryo-EM, discover Hsp15/RqcP as an essential factor, and reveal that C-terminal tailing follows similar principles to canonical translation elongation.

KW - Hsp15

KW - RQC

KW - RqcH

KW - RqcP

KW - SsrA

KW - alanine tailing

KW - cryo-EM

KW - ribosome-associated quality control

KW - ribosomes

KW - translation elongation

UR - http://www.scopus.com/inward/record.url?scp=85097872385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85097872385&partnerID=8YFLogxK

U2 - 10.1016/j.molcel.2020.11.001

DO - 10.1016/j.molcel.2020.11.001

M3 - Article

C2 - 33259811

AN - SCOPUS:85097872385

SN - 1097-2765

VL - 81

SP - 104-114.e6

JO - Molecular cell

JF - Molecular cell

IS - 1

ER -

Mimicry of Canonical Translation Elongation Underlies Alanine Tail Synthesis in RQC

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this