TY - JOUR
T1 - Midgut transcriptome response to a Cry toxin in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)
AU - Lei, Yanyuan
AU - Zhu, Xun
AU - Xie, Wen
AU - Wu, Qingjun
AU - Wang, Shaoli
AU - Guo, Zhaojiang
AU - Xu, Baoyun
AU - Li, Xianchun
AU - Zhou, Xuguo
AU - Zhang, Youjun
N1 - Funding Information:
We thank the Beijing Genomics Institute at Shenzhen for the assistance in original data processing and related bioinformatics analysis. This work was supported by the National Science and Technology support task ( 2012BAD19B06 ), the National High Technology Research and Development Program of China (863 Program) ( 2012AA101502 ), the Special Fund for Agro-scientific Research in the Public Interest ( 201103021 ), and the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables .
PY - 2014/1/1
Y1 - 2014/1/1
N2 - To investigate the response of Plutella xylostella transcriptome in defending against a Bt toxin, high-throughput RNA-sequencing was carried out to examine Cry1Ac-susceptible and -resistant strains. The comparative analysis indentified over 2900 differentially expressed unigenes (DEUs) between these two strains. Gene Ontology analysis placed these unigenes primarily into cell, cell part, organelle, binding, catalytic, cellular process, metabolic process, and response to stimulus categories. Based on pathway analyses, DEUs were enriched in oxidoreductase activity and membrane lipid metabolic processes, and they were also significantly enriched in pathways related to the metabolic and biosynthesis of secondary metabolites. Most of the unigenes involved in the metabolic pathway were up-regulated in resistant strains. Within the ABC transporter pathway, majority of the down-regulated unigenes belong to ABCC2 and ABCC10, respectively, while up-regulated unigenes were mainly categorized as ABCG2. Furthermore, two aminopeptidases, and four cadherins encoding genes were significantly elevated as well. This study provides a transcriptome foundation for the identification and functional characterization of genes involved in the Bt resistance in an agriculturally important insect pest, P. xylostella.
AB - To investigate the response of Plutella xylostella transcriptome in defending against a Bt toxin, high-throughput RNA-sequencing was carried out to examine Cry1Ac-susceptible and -resistant strains. The comparative analysis indentified over 2900 differentially expressed unigenes (DEUs) between these two strains. Gene Ontology analysis placed these unigenes primarily into cell, cell part, organelle, binding, catalytic, cellular process, metabolic process, and response to stimulus categories. Based on pathway analyses, DEUs were enriched in oxidoreductase activity and membrane lipid metabolic processes, and they were also significantly enriched in pathways related to the metabolic and biosynthesis of secondary metabolites. Most of the unigenes involved in the metabolic pathway were up-regulated in resistant strains. Within the ABC transporter pathway, majority of the down-regulated unigenes belong to ABCC2 and ABCC10, respectively, while up-regulated unigenes were mainly categorized as ABCG2. Furthermore, two aminopeptidases, and four cadherins encoding genes were significantly elevated as well. This study provides a transcriptome foundation for the identification and functional characterization of genes involved in the Bt resistance in an agriculturally important insect pest, P. xylostella.
KW - ABC transporters
KW - Bt resistance
KW - Cry1Ac
KW - Plutella xylostella
KW - RNA-seq
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U2 - 10.1016/j.gene.2013.09.091
DO - 10.1016/j.gene.2013.09.091
M3 - Article
C2 - 24120626
AN - SCOPUS:84887238534
SN - 0378-1119
VL - 533
SP - 180
EP - 187
JO - Gene
JF - Gene
IS - 1
ER -