Microanatomic relationships between CD8+ cells and HIV-1-producing cells in human lymphoid tissue in vivo

Joy M. Folkvord, Deborah M. Anderson, Jyoti Arya, Samantha MaWhinney, Elizabeth Connick

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Objective: Host immune responses are unable to fully suppress HIV-1 replication in lymphoid tissues. Microanatomic relationships between HIV-1-producing cells and CD8+ cells in lymphoid tissues were analyzed to determine whether there was evidence for an immune privileged site or impaired recognition of virus-producing cells. Methods: CD8+ cell phenotypes were determined on disaggregated inguinal lymph node cells by flow cytometry for seven untreated HIV-1-infected subjects. Microanatomic relationships between HIV-1-producing cells and CD8+ cells were analyzed in lymph node sections from 15 HIV-1-infected individuals using in situ hybridization and immunohistochemical staining. Results: Most (median, 96%) lymph node CD8+ cells coexpressed CD3. Frequencies of virus-producing cells detected by in situ hybridization correlated with plasma HIV-1 RNA concentration (Spearman p = 0.70; p = .02; n = 11). The percentage of lymph node cells adjacent to virus-producing cells that were CD8+ (median, 29%) was not statistically different from the percentage of CD8+ cells in lymphoid tissue overall (median, 34%; p = .09). Conclusions: Multiple explanations could account for the observation that CD8+ cells do not preferentially accumulate around virus-producing cells including the possibility that HIV-1-specific CD8+ cells cannot recognize virus-producing cells. Further studies are necessary to determine whether HIV-1-specific CD8+ T cells aggregate around virus-producing cells in lymphoid tissue.

Original languageEnglish (US)
Pages (from-to)469-476
Number of pages8
JournalJournal of Acquired Immune Deficiency Syndromes
Volume32
Issue number5
DOIs
StatePublished - Apr 15 2003
Externally publishedYes

Keywords

  • CD8T lymphocytes
  • Cytotoxic T lymphocytes
  • HIV infections
  • Lymph nodes
  • Lymphoid tissue

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)

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