Mice deleted for heart-type cytochrome c oxidase subunit 7a1 develop dilated cardiomyopathy

Maik Hüttemann; Scott Klewer; Icksoo Lee; Alena Pecinova; Petr Pecina; Jenney Liu; Michael Lee; Jeffrey W. Doan; Douglas F Larson; Elise Slack; Bita Maghsoodi; Robert P. Erickson; Lawrence I. Grossman

doi:10.1016/j.mito.2011.11.002

Mice deleted for heart-type cytochrome c oxidase subunit 7a1 develop dilated cardiomyopathy

Maik Hüttemann, Scott Klewer, Icksoo Lee, Alena Pecinova, Petr Pecina, Jenney Liu, Michael Lee, Jeffrey W. Doan, Douglas F Larson, Elise Slack, Bita Maghsoodi, Robert P. Erickson, Lawrence I. Grossman

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Subunit 7a of mouse cytochrome c oxidase (Cox) displays a contractile muscle-specific isoform, Cox7a1, that is the major cardiac form. To gain insight into the role of this isoform, we have produced a new knockout mouse line that lacks Cox7a1. We show that homozygous and heterozygous Cox7a1 knockout mice, although viable, have reduced Cox activity and develop a dilated cardiomyopathy at 6. weeks of age. Surprisingly, the cardiomyopathy improves and stabilizes by 6. months of age. Cox7a1 knockout mice incorporate more of the "liver-type" isoform Cox7a2 into the cardiac Cox holoenzyme and, also surprisingly, have higher tissue ATP levels.

Original language	English (US)
Pages (from-to)	294-304
Number of pages	11
Journal	Mitochondrion
Volume	12
Issue number	2
DOIs	https://doi.org/10.1016/j.mito.2011.11.002
State	Published - Mar 2012

Keywords

Dilated cardiomyopathy
Electron transport chain
Gene knockout
Mitochondria
Oxidative phosphorylation

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Cell Biology

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10.1016/j.mito.2011.11.002

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Mice deleted for heart-type cytochrome c oxidase subunit 7a1 develop dilated cardiomyopathy. / Hüttemann, Maik; Klewer, Scott; Lee, Icksoo; Pecinova, Alena; Pecina, Petr; Liu, Jenney; Lee, Michael; Doan, Jeffrey W.; Larson, Douglas F; Slack, Elise; Maghsoodi, Bita; Erickson, Robert P.; Grossman, Lawrence I.

In: Mitochondrion, Vol. 12, No. 2, 03.2012, p. 294-304.

Research output: Contribution to journal › Article › peer-review

Hüttemann, Maik ; Klewer, Scott ; Lee, Icksoo ; Pecinova, Alena ; Pecina, Petr ; Liu, Jenney ; Lee, Michael ; Doan, Jeffrey W. ; Larson, Douglas F ; Slack, Elise ; Maghsoodi, Bita ; Erickson, Robert P. ; Grossman, Lawrence I. / Mice deleted for heart-type cytochrome c oxidase subunit 7a1 develop dilated cardiomyopathy. In: Mitochondrion. 2012 ; Vol. 12, No. 2. pp. 294-304.

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note = "Funding Information: This work was supported by a grant supplement from the National Institutes of Health (GM48517; LIG), the Center for Molecular Medicine and Genetics, Wayne State University, Detroit (MH), and a grant from the National Institutes of Health (GM089900; MH). ",

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AU - Larson, Douglas F

AU - Slack, Elise

AU - Maghsoodi, Bita

AU - Erickson, Robert P.

AU - Grossman, Lawrence I.

N1 - Funding Information: This work was supported by a grant supplement from the National Institutes of Health (GM48517; LIG), the Center for Molecular Medicine and Genetics, Wayne State University, Detroit (MH), and a grant from the National Institutes of Health (GM089900; MH).

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Mice deleted for heart-type cytochrome c oxidase subunit 7a1 develop dilated cardiomyopathy

Abstract

Keywords

ASJC Scopus subject areas

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