Methylprednisolone accelerates the ontogeny of sodium-taurocholate cotransport in rat ileal brush border membranes

J. A. Barnard, F. K. Ghishan

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


The effect of methylprednisolone on the postnatal maturation of taurocholate transport was studied by using isolated ileal brush border membrane vesicles. Vesicles were prepared from 14-day-old control, 14-day-old methylprednisolone-treated, and untreated 21-day-old rats. Methylprednisolone treatment resulted in a significant stimulation of taurocholate uptake by an inwardly directed Na+ gradient when compared with a choline gradient incubation. These differences occurred at 20 seconds and 1, 2, and 5 minutes of incubation (P < 0.05). In 14-day-old controls, uptake was similar for Na+ and choline gradients. A plot of active uptake velocity vs. taurocholate concentration (0.1 to 1.0 mmol/L) in 14-day-old controls was linear and approached the abscissa, indicating the absence of active transport. Plots for methylprednisolone-treated rats showed saturability. An inwardly directed Na+ gradient stimulated initial taurocholate uptake rates by twofold at 37 ° C (P < 0.01), but not at 4 °C. Glycocholate and glycodeoxycholate inhibited Na+-stimulated taurocholate uptake by 50% (P < 0.01) and 20% (P < 0.05), respectively. These data indicate that pharmacologic doses of methylprednisolone accelerate the postnatal acquisition of Na+-dependent taurocholate cotransport in rat ileal brush border membranes.

Original languageEnglish (US)
Pages (from-to)549-555
Number of pages7
JournalThe Journal of Laboratory and Clinical Medicine
Issue number6
StatePublished - Dec 1986

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


Dive into the research topics of 'Methylprednisolone accelerates the ontogeny of sodium-taurocholate cotransport in rat ileal brush border membranes'. Together they form a unique fingerprint.

Cite this