Methylation of the alpha-fetoprotein gene in isogenic rat hepatoma and liver cell lines

Keiko Sakuma; Jeffry R. Cook; Catharine L. Smith; Jen Fu Chiu

doi:10.1016/0006-291X(87)91374-X

Methylation of the alpha-fetoprotein gene in isogenic rat hepatoma and liver cell lines

Keiko Sakuma, Jeffry R. Cook, Catharine L. Smith, Jen Fu Chiu

Research output: Contribution to journal › Article › peer-review

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Abstract

We have found that DNA methylation is inversely correlated with alpha-fetoprotein (AFP) gene expression in a series of isogenic rat hepatoma cell lines. The 5′ end of the gene is extensively demethylated in AFP-producing cells and is highly methylated in cell lines which do not produce AFP. Glucocorticoid affects markedly the synthesis of AFP in the hepatoma cells. However, methylation patterns of cell lines which were treated with dexamethasone were not different from those of control cells, indicating that glucocorticoid action on AFP gene expression does not alter DNA methylation in this region of the gene.

Original language	English (US)
Pages (from-to)	447-453
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	143
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(87)91374-X
State	Published - Mar 13 1987
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Methylation of the alpha-fetoprotein gene in isogenic rat hepatoma and liver cell lines",

abstract = "We have found that DNA methylation is inversely correlated with alpha-fetoprotein (AFP) gene expression in a series of isogenic rat hepatoma cell lines. The 5′ end of the gene is extensively demethylated in AFP-producing cells and is highly methylated in cell lines which do not produce AFP. Glucocorticoid affects markedly the synthesis of AFP in the hepatoma cells. However, methylation patterns of cell lines which were treated with dexamethasone were not different from those of control cells, indicating that glucocorticoid action on AFP gene expression does not alter DNA methylation in this region of the gene.",

author = "Keiko Sakuma and Cook, {Jeffry R.} and Smith, {Catharine L.} and Chiu, {Jen Fu}",

note = "Funding Information: This work was supported by NIH grant CA 25098. the assistance of Helen Plumley during the preparation",

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T1 - Methylation of the alpha-fetoprotein gene in isogenic rat hepatoma and liver cell lines

AU - Sakuma, Keiko

AU - Cook, Jeffry R.

AU - Smith, Catharine L.

AU - Chiu, Jen Fu

N1 - Funding Information: This work was supported by NIH grant CA 25098. the assistance of Helen Plumley during the preparation

PY - 1987/3/13

Y1 - 1987/3/13

N2 - We have found that DNA methylation is inversely correlated with alpha-fetoprotein (AFP) gene expression in a series of isogenic rat hepatoma cell lines. The 5′ end of the gene is extensively demethylated in AFP-producing cells and is highly methylated in cell lines which do not produce AFP. Glucocorticoid affects markedly the synthesis of AFP in the hepatoma cells. However, methylation patterns of cell lines which were treated with dexamethasone were not different from those of control cells, indicating that glucocorticoid action on AFP gene expression does not alter DNA methylation in this region of the gene.

AB - We have found that DNA methylation is inversely correlated with alpha-fetoprotein (AFP) gene expression in a series of isogenic rat hepatoma cell lines. The 5′ end of the gene is extensively demethylated in AFP-producing cells and is highly methylated in cell lines which do not produce AFP. Glucocorticoid affects markedly the synthesis of AFP in the hepatoma cells. However, methylation patterns of cell lines which were treated with dexamethasone were not different from those of control cells, indicating that glucocorticoid action on AFP gene expression does not alter DNA methylation in this region of the gene.

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Methylation of the alpha-fetoprotein gene in isogenic rat hepatoma and liver cell lines

Abstract

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