Methods for phospholipid extraction in candida albicans: An extraction method with high efficacy

F. Mirbod; S. Mori; Y. Nozawa

doi:10.1080/02681219380000521

Methods for phospholipid extraction in candida albicans: An extraction method with high efficacy

F. Mirbod, S. Mori, Y. Nozawa

Research output: Contribution to journal › Article › peer-review

Abstract

Phospholipid composition of Candida albicans was examined and compared using the three established methods for lipid extraction (Folch, Bligh-Dyer and Angus-Lester methods). The highest yield of total phospholipids was obtained by methanol pre-treatment prior to extraction with Folch solution. The percentage distribution of individual phospholipids showed a much higher level of phosphatidylcholine following extraction by the modified Folch method compared with extraction by the other methods.

Original language	English (US)
Pages (from-to)	405-409
Number of pages	5
Journal	Medical mycology
Volume	31
Issue number	5
DOIs	https://doi.org/10.1080/02681219380000521
State	Published - 1993
Externally published	Yes

ASJC Scopus subject areas

Infectious Diseases

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10.1080/02681219380000521

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title = "Methods for phospholipid extraction in candida albicans: An extraction method with high efficacy",

abstract = "Phospholipid composition of Candida albicans was examined and compared using the three established methods for lipid extraction (Folch, Bligh-Dyer and Angus-Lester methods). The highest yield of total phospholipids was obtained by methanol pre-treatment prior to extraction with Folch solution. The percentage distribution of individual phospholipids showed a much higher level of phosphatidylcholine following extraction by the modified Folch method compared with extraction by the other methods.",

author = "F. Mirbod and S. Mori and Y. Nozawa",

year = "1993",

doi = "10.1080/02681219380000521",

language = "English (US)",

volume = "31",

pages = "405--409",

journal = "Medical mycology",

issn = "1369-3786",

publisher = "Informa Healthcare",

number = "5",

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TY - JOUR

T1 - Methods for phospholipid extraction in candida albicans

T2 - An extraction method with high efficacy

AU - Mirbod, F.

AU - Mori, S.

AU - Nozawa, Y.

PY - 1993

Y1 - 1993

N2 - Phospholipid composition of Candida albicans was examined and compared using the three established methods for lipid extraction (Folch, Bligh-Dyer and Angus-Lester methods). The highest yield of total phospholipids was obtained by methanol pre-treatment prior to extraction with Folch solution. The percentage distribution of individual phospholipids showed a much higher level of phosphatidylcholine following extraction by the modified Folch method compared with extraction by the other methods.

AB - Phospholipid composition of Candida albicans was examined and compared using the three established methods for lipid extraction (Folch, Bligh-Dyer and Angus-Lester methods). The highest yield of total phospholipids was obtained by methanol pre-treatment prior to extraction with Folch solution. The percentage distribution of individual phospholipids showed a much higher level of phosphatidylcholine following extraction by the modified Folch method compared with extraction by the other methods.

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U2 - 10.1080/02681219380000521

DO - 10.1080/02681219380000521

M3 - Article

AN - SCOPUS:84907126611

SN - 1369-3786

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JO - Medical mycology

JF - Medical mycology

IS - 5

ER -

Methods for phospholipid extraction in candida albicans: An extraction method with high efficacy

Abstract

ASJC Scopus subject areas

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