Methods for concentrating viruses from large volumes of estuarine water on pleated membranes

P. Payment; C. P. Gerba; C. Wallis; J. L. Melnick

doi:10.1016/0043-1354(76)90024-5

Methods for concentrating viruses from large volumes of estuarine water on pleated membranes

P. Payment, C. P. Gerba, C. Wallis, J. L. Melnick

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The efficiency of cartridge filters with pleated membranes was determined for the recovery of poliovirus from large volumes of seawater. Flow rates of about 6 gal min^-1 were obtained when a combination of a 3- and a 0.45-μm pleated filter were incorporated into the Wallis-Melnick virus concentrator. The filters adsorbed poliovirus when water was acidified at pH 3.5 and aluminum chloride added at a final concentration of 0.0015 m. The filters retained their capacity to adsorb virus particles, even after 600 gal (22681.) of very turbid estuarine water had been filtered. Viruses were eluted from the pleated filters with pH 11.5 glycine buffer and reconcentrated by precipitation with ferric chloride or aluminum chloride. Virus from 100-gal samples was concentrated into a final eluate volume of 20-100 ml, with virus recovery of about 50% being achieved in the material that had been concentrated 20,000-100,000 times.

Original language	English (US)
Pages (from-to)	893-896
Number of pages	4
Journal	Water research
Volume	10
Issue number	10
DOIs	https://doi.org/10.1016/0043-1354(76)90024-5
State	Published - 1976
Externally published	Yes

ASJC Scopus subject areas

Water Science and Technology
Ecological Modeling
Pollution
Waste Management and Disposal
Environmental Engineering
Civil and Structural Engineering

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10.1016/0043-1354(76)90024-5

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title = "Methods for concentrating viruses from large volumes of estuarine water on pleated membranes",

abstract = "The efficiency of cartridge filters with pleated membranes was determined for the recovery of poliovirus from large volumes of seawater. Flow rates of about 6 gal min-1 were obtained when a combination of a 3- and a 0.45-μm pleated filter were incorporated into the Wallis-Melnick virus concentrator. The filters adsorbed poliovirus when water was acidified at pH 3.5 and aluminum chloride added at a final concentration of 0.0015 m. The filters retained their capacity to adsorb virus particles, even after 600 gal (22681.) of very turbid estuarine water had been filtered. Viruses were eluted from the pleated filters with pH 11.5 glycine buffer and reconcentrated by precipitation with ferric chloride or aluminum chloride. Virus from 100-gal samples was concentrated into a final eluate volume of 20-100 ml, with virus recovery of about 50% being achieved in the material that had been concentrated 20,000-100,000 times.",

author = "P. Payment and Gerba, {C. P.} and C. Wallis and Melnick, {J. L.}",

note = "Funding Information: Acknowledgements This study was supported in part by research project R-802736-01 from the Environment Protection Agency, by the Carborundum Company, Niagara Falls, New York. and by the Oceanic and Atmospheric Administration's Office of Sea Grants, Department of Commerce, through institutional grant 2-35213 to Texas A&M University. Dr. Payment is the recipient of an MRC (Canada) fellowship.",

year = "1976",

doi = "10.1016/0043-1354(76)90024-5",

language = "English (US)",

volume = "10",

pages = "893--896",

journal = "Water research",

issn = "0043-1354",

publisher = "Elsevier Limited",

number = "10",

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TY - JOUR

T1 - Methods for concentrating viruses from large volumes of estuarine water on pleated membranes

AU - Payment, P.

AU - Gerba, C. P.

AU - Wallis, C.

AU - Melnick, J. L.

N1 - Funding Information: Acknowledgements This study was supported in part by research project R-802736-01 from the Environment Protection Agency, by the Carborundum Company, Niagara Falls, New York. and by the Oceanic and Atmospheric Administration's Office of Sea Grants, Department of Commerce, through institutional grant 2-35213 to Texas A&M University. Dr. Payment is the recipient of an MRC (Canada) fellowship.

PY - 1976

Y1 - 1976

N2 - The efficiency of cartridge filters with pleated membranes was determined for the recovery of poliovirus from large volumes of seawater. Flow rates of about 6 gal min-1 were obtained when a combination of a 3- and a 0.45-μm pleated filter were incorporated into the Wallis-Melnick virus concentrator. The filters adsorbed poliovirus when water was acidified at pH 3.5 and aluminum chloride added at a final concentration of 0.0015 m. The filters retained their capacity to adsorb virus particles, even after 600 gal (22681.) of very turbid estuarine water had been filtered. Viruses were eluted from the pleated filters with pH 11.5 glycine buffer and reconcentrated by precipitation with ferric chloride or aluminum chloride. Virus from 100-gal samples was concentrated into a final eluate volume of 20-100 ml, with virus recovery of about 50% being achieved in the material that had been concentrated 20,000-100,000 times.

AB - The efficiency of cartridge filters with pleated membranes was determined for the recovery of poliovirus from large volumes of seawater. Flow rates of about 6 gal min-1 were obtained when a combination of a 3- and a 0.45-μm pleated filter were incorporated into the Wallis-Melnick virus concentrator. The filters adsorbed poliovirus when water was acidified at pH 3.5 and aluminum chloride added at a final concentration of 0.0015 m. The filters retained their capacity to adsorb virus particles, even after 600 gal (22681.) of very turbid estuarine water had been filtered. Viruses were eluted from the pleated filters with pH 11.5 glycine buffer and reconcentrated by precipitation with ferric chloride or aluminum chloride. Virus from 100-gal samples was concentrated into a final eluate volume of 20-100 ml, with virus recovery of about 50% being achieved in the material that had been concentrated 20,000-100,000 times.

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DO - 10.1016/0043-1354(76)90024-5

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AN - SCOPUS:0017139115

SN - 0043-1354

VL - 10

SP - 893

EP - 896

JO - Water research

JF - Water research

IS - 10

ER -

Methods for concentrating viruses from large volumes of estuarine water on pleated membranes

Abstract

ASJC Scopus subject areas

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