Methanogenesis in granular sludge exposed to oxygen

Mario T. Kato; Jim A. Field; Gatze Lettinga

doi:10.1111/j.1574-6968.1993.tb06592.x

Methanogenesis in granular sludge exposed to oxygen

Mario T. Kato, Jim A. Field, Gatze Lettinga

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43 Scopus citations

Abstract

Substrate competition between methanogenic and facultative bacteria under highly aerobic conditions was investigated in batch experiments. Natural mixed cultures of anaerobic bacteria immobilized in granular sludge were able to concurrently utilize oxygen and produce methane when supplied with ethanol as substrate. The most oxygen tolerant sludge converted 3 to 25% of substrate chemical oxygen demand to methane after 3 days while 23 to 2 mg 1^-1 of dissolved oxygen were present in the media. The tolerance of methanogens to oxygen and their coexistence with facultative bacteria were evident even after long periods of oxygen exposure. Eventually, methane oxidizing bacteria developed in the co-culture. The consumption of oxygen by facultative bacteria, creating anaerobic microniches inside the granules, is hypothesized to protect the methanogens.

Original language	English (US)
Pages (from-to)	317-323
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	114
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-6968.1993.tb06592.x
State	Published - Dec 15 1993
Externally published	Yes

Keywords

Anaerobic methanogenic and aerobic facultative bacteria
Co-culture
Methanogens
Substrate competition

ASJC Scopus subject areas

General Medicine

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10.1111/j.1574-6968.1993.tb06592.x

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@article{76a99e07621846df9d12b74a67db7034,

title = "Methanogenesis in granular sludge exposed to oxygen",

abstract = "Substrate competition between methanogenic and facultative bacteria under highly aerobic conditions was investigated in batch experiments. Natural mixed cultures of anaerobic bacteria immobilized in granular sludge were able to concurrently utilize oxygen and produce methane when supplied with ethanol as substrate. The most oxygen tolerant sludge converted 3 to 25% of substrate chemical oxygen demand to methane after 3 days while 23 to 2 mg 1-1 of dissolved oxygen were present in the media. The tolerance of methanogens to oxygen and their coexistence with facultative bacteria were evident even after long periods of oxygen exposure. Eventually, methane oxidizing bacteria developed in the co-culture. The consumption of oxygen by facultative bacteria, creating anaerobic microniches inside the granules, is hypothesized to protect the methanogens.",

keywords = "Anaerobic methanogenic and aerobic facultative bacteria, Co-culture, Methanogens, Substrate competition",

author = "Kato, {Mario T.} and Field, {Jim A.} and Gatze Lettinga",

note = "Funding Information: Financial aid to M.T. Kato from the Ministry of Education/CAPES and Federal University of Pernambuco, Recife, Brazil, is gratefully acknowledged. We would also like to thank L. Florencio for offering her help in some of the experimental work, J. van der Laan and M. de Wit for the technical assistance with the chromatographs.",

year = "1993",

month = dec,

day = "15",

doi = "10.1111/j.1574-6968.1993.tb06592.x",

language = "English (US)",

volume = "114",

pages = "317--323",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

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TY - JOUR

T1 - Methanogenesis in granular sludge exposed to oxygen

AU - Kato, Mario T.

AU - Field, Jim A.

AU - Lettinga, Gatze

N1 - Funding Information: Financial aid to M.T. Kato from the Ministry of Education/CAPES and Federal University of Pernambuco, Recife, Brazil, is gratefully acknowledged. We would also like to thank L. Florencio for offering her help in some of the experimental work, J. van der Laan and M. de Wit for the technical assistance with the chromatographs.

PY - 1993/12/15

Y1 - 1993/12/15

N2 - Substrate competition between methanogenic and facultative bacteria under highly aerobic conditions was investigated in batch experiments. Natural mixed cultures of anaerobic bacteria immobilized in granular sludge were able to concurrently utilize oxygen and produce methane when supplied with ethanol as substrate. The most oxygen tolerant sludge converted 3 to 25% of substrate chemical oxygen demand to methane after 3 days while 23 to 2 mg 1-1 of dissolved oxygen were present in the media. The tolerance of methanogens to oxygen and their coexistence with facultative bacteria were evident even after long periods of oxygen exposure. Eventually, methane oxidizing bacteria developed in the co-culture. The consumption of oxygen by facultative bacteria, creating anaerobic microniches inside the granules, is hypothesized to protect the methanogens.

AB - Substrate competition between methanogenic and facultative bacteria under highly aerobic conditions was investigated in batch experiments. Natural mixed cultures of anaerobic bacteria immobilized in granular sludge were able to concurrently utilize oxygen and produce methane when supplied with ethanol as substrate. The most oxygen tolerant sludge converted 3 to 25% of substrate chemical oxygen demand to methane after 3 days while 23 to 2 mg 1-1 of dissolved oxygen were present in the media. The tolerance of methanogens to oxygen and their coexistence with facultative bacteria were evident even after long periods of oxygen exposure. Eventually, methane oxidizing bacteria developed in the co-culture. The consumption of oxygen by facultative bacteria, creating anaerobic microniches inside the granules, is hypothesized to protect the methanogens.

KW - Anaerobic methanogenic and aerobic facultative bacteria

KW - Co-culture

KW - Methanogens

KW - Substrate competition

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U2 - 10.1111/j.1574-6968.1993.tb06592.x

DO - 10.1111/j.1574-6968.1993.tb06592.x

M3 - Article

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SN - 0378-1097

VL - 114

SP - 317

EP - 323

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 3

ER -

Methanogenesis in granular sludge exposed to oxygen

Abstract

Keywords

ASJC Scopus subject areas

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