Metalloproteinases 2 and -9 activity during promotion and progression stages of rat liver carcinogenesis

Kelly Silva Furtado, Paulo Wagner Pires, Luis Antonio Justulin, Maria Aparecida Marchesan Rodrigues, Sergio Luis Felisbino, Luis Fernando Barbisan

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Activity of metalloproteinases 2 and 9 (MMP-2 and 9) during promotion and progression of rat liver carcinogenesis was investigated in a modified resistant hepatocyte model. Development of preneoplastic liver lesions positive for glutathione S-transferase 7-7-(GST-P 7-7-positive PNL) and tumors besides hepatocytes positive for proliferating cell nuclear antigen (PCNA) were quantified and compared to MMP-2 and-9 activity using gelatin zymography. Marked increases in GST-P 7-7-positive PNL development, PCNA labeling indices, MMP-2 (pro, intermediate and active forms) and pro-MMP-9 activity were observed after proliferative stimulus induced by 2-acetylaminofluorene (2-AAF) exposure cycles. After 2-AAF withdrawal, increase in MMP-2 activity was detected only in neoplastic mixed lesions, whereas active MMP-9 was increased in both PLN and neoplastic tissues. Our findings suggest that MMP-2 may be associated with proliferative events induced by 2-AAF rather than with selective growth of PNL and that MMP-9 could be associated with progression of PNL and neoplastic mixed lesions.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalJournal of Molecular Histology
Issue number1
StatePublished - Feb 2009
Externally publishedYes


  • Metalloproteinases
  • Preneoplastic and neoplastic lesions
  • Rat liver carcinogenesis
  • Resistant hepatocyte model

ASJC Scopus subject areas

  • Histology
  • Physiology
  • Cell Biology


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