Metabolism of arachidonic acid by isolated rabbit ciliary epithelium

Kara L. King; Nicholas A. Delamere; Stephen C. Csukas; William M. Pierce

doi:10.1016/0014-4835(92)90187-W

Metabolism of arachidonic acid by isolated rabbit ciliary epithelium

Kara L. King, Nicholas A. Delamere, Stephen C. Csukas, William M. Pierce

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We examined the ability of rabbit ciliary epithelium to metabolize arachidonic acid in vitro. The epithelium was homogenized and incubated with ¹⁴C-labeled arachidonic acid. ¹⁴C-labeled metabolites were extracted and then separated by thin layer chromatography. The range of arachidonic acid metabolites synthesized by ciliary epithelium was compared to the metabolites generated by rabbit irisciliary body. Ciliary epithelium produced substantial amounts of arachidonic acid metabolites that comigrated with 5-HETE and 12-HETE. Authenticity of the 12-HETE produced by ciliary epithelium was confirmed by gas chromatography/mass spectrometry. The ciliary epithelium generated only small amounts of the cyclooxygenase products, PGF_2α, PGE₂, PGD₂ and 6k-PGF_1α. In contrast, the iris-ciliary body produced large amounts of cyclooxygenase products such as PGF_2α and PGD₂. The ability of the ciliary epithelium to generate 12-HETE is noteworthy since 12(R)-HETE is known to be capable of lowering intraocular pressure.

Original language	English (US)
Pages (from-to)	235-241
Number of pages	7
Journal	Experimental eye research
Volume	55
Issue number	2
DOIs	https://doi.org/10.1016/0014-4835(92)90187-W
State	Published - Aug 1992
Externally published	Yes

Keywords

HETES
arachidonic acid
ciliary epithelium
iris-ciliary body
prostaglandins
rabbit

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.1016/0014-4835(92)90187-W

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title = "Metabolism of arachidonic acid by isolated rabbit ciliary epithelium",

abstract = "We examined the ability of rabbit ciliary epithelium to metabolize arachidonic acid in vitro. The epithelium was homogenized and incubated with 14C-labeled arachidonic acid. 14C-labeled metabolites were extracted and then separated by thin layer chromatography. The range of arachidonic acid metabolites synthesized by ciliary epithelium was compared to the metabolites generated by rabbit irisciliary body. Ciliary epithelium produced substantial amounts of arachidonic acid metabolites that comigrated with 5-HETE and 12-HETE. Authenticity of the 12-HETE produced by ciliary epithelium was confirmed by gas chromatography/mass spectrometry. The ciliary epithelium generated only small amounts of the cyclooxygenase products, PGF2α, PGE2, PGD2 and 6k-PGF1α. In contrast, the iris-ciliary body produced large amounts of cyclooxygenase products such as PGF2α and PGD2. The ability of the ciliary epithelium to generate 12-HETE is noteworthy since 12(R)-HETE is known to be capable of lowering intraocular pressure.",

keywords = "HETES, arachidonic acid, ciliary epithelium, iris-ciliary body, prostaglandins, rabbit",

author = "King, {Kara L.} and Delamere, {Nicholas A.} and Csukas, {Stephen C.} and Pierce, {William M.}",

note = "Funding Information: This work was supported by USPHS Research Grants .No. EY06915 and EYO6918, the Kentucky Lions Eye Foun-dation, and an unrestricted grant from Research to Prevent Blindness, Inc. We wish to thank Drs Parimal Bhattacherjee,",

year = "1992",

month = aug,

doi = "10.1016/0014-4835(92)90187-W",

language = "English (US)",

volume = "55",

pages = "235--241",

journal = "Experimental eye research",

issn = "0014-4835",

publisher = "Academic Press",

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T1 - Metabolism of arachidonic acid by isolated rabbit ciliary epithelium

AU - King, Kara L.

AU - Delamere, Nicholas A.

AU - Csukas, Stephen C.

AU - Pierce, William M.

N1 - Funding Information: This work was supported by USPHS Research Grants .No. EY06915 and EYO6918, the Kentucky Lions Eye Foun-dation, and an unrestricted grant from Research to Prevent Blindness, Inc. We wish to thank Drs Parimal Bhattacherjee,

PY - 1992/8

Y1 - 1992/8

N2 - We examined the ability of rabbit ciliary epithelium to metabolize arachidonic acid in vitro. The epithelium was homogenized and incubated with 14C-labeled arachidonic acid. 14C-labeled metabolites were extracted and then separated by thin layer chromatography. The range of arachidonic acid metabolites synthesized by ciliary epithelium was compared to the metabolites generated by rabbit irisciliary body. Ciliary epithelium produced substantial amounts of arachidonic acid metabolites that comigrated with 5-HETE and 12-HETE. Authenticity of the 12-HETE produced by ciliary epithelium was confirmed by gas chromatography/mass spectrometry. The ciliary epithelium generated only small amounts of the cyclooxygenase products, PGF2α, PGE2, PGD2 and 6k-PGF1α. In contrast, the iris-ciliary body produced large amounts of cyclooxygenase products such as PGF2α and PGD2. The ability of the ciliary epithelium to generate 12-HETE is noteworthy since 12(R)-HETE is known to be capable of lowering intraocular pressure.

AB - We examined the ability of rabbit ciliary epithelium to metabolize arachidonic acid in vitro. The epithelium was homogenized and incubated with 14C-labeled arachidonic acid. 14C-labeled metabolites were extracted and then separated by thin layer chromatography. The range of arachidonic acid metabolites synthesized by ciliary epithelium was compared to the metabolites generated by rabbit irisciliary body. Ciliary epithelium produced substantial amounts of arachidonic acid metabolites that comigrated with 5-HETE and 12-HETE. Authenticity of the 12-HETE produced by ciliary epithelium was confirmed by gas chromatography/mass spectrometry. The ciliary epithelium generated only small amounts of the cyclooxygenase products, PGF2α, PGE2, PGD2 and 6k-PGF1α. In contrast, the iris-ciliary body produced large amounts of cyclooxygenase products such as PGF2α and PGD2. The ability of the ciliary epithelium to generate 12-HETE is noteworthy since 12(R)-HETE is known to be capable of lowering intraocular pressure.

KW - HETES

KW - arachidonic acid

KW - ciliary epithelium

KW - iris-ciliary body

KW - prostaglandins

KW - rabbit

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ER -

Metabolism of arachidonic acid by isolated rabbit ciliary epithelium

Abstract

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