TY - JOUR
T1 - Metabolic interactions in a bacterial co-culture accelerate phenanthrene degradation
AU - Sun, Shanshan
AU - Wang, Haizhen
AU - Yan, Kang
AU - Lou, Jun
AU - Ding, Jiahui
AU - Snyder, Shane A.
AU - Wu, Laosheng
AU - Xu, Jianming
N1 - Funding Information:
This work was supported by the National Key Research and Development Program of China ( 2019YFC1803704 ); and the National Natural Science Foundation of China ( 41771344 ).
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/2/5
Y1 - 2021/2/5
N2 - A highly eff ;ective phenanthrene (PHE)-degrading co-culture containing Rhodococcus sp. WB9 and Mycobacterium sp. WY10 was constructed and completely degraded 100 mg L−1 PHE within 36 h, showing improved degradation rate compared to their monocultures. In the co-culture, strain WY10 played a predominant role in PHE degradation. 1-hydroxy-2-naphthoic acid was an end-product of PHE degradation by strain WB9 and accumulated in the culture medium to serve as a substrate for strain WY10 growth, thereby accelerating PHE degradation. In turn, strain WY10 degraded PHE and 1-hydroxy-2-naphthoic acid intracellularly to form phthalate and protocatechuate that were exported to the culture medium through efflux transporters. However, strain WY10 cannot take up extracellular phthalate due to the absence of phthalate transporters, restricting phthalate degradation and PHE mineralization. In the co-culture, phthalate and protocatechuate accumulated in the culture medium were taken up and degraded towards TCA cycle by strain WB9. Therefore, the metabolic cross-feeding of strains WB9 and WY10 accelerated PHE degradation and mineralization. These findings exhibiting the synergistic degradation of PHE in the bacterial co-culture will facilitate its bioremediation application.
AB - A highly eff ;ective phenanthrene (PHE)-degrading co-culture containing Rhodococcus sp. WB9 and Mycobacterium sp. WY10 was constructed and completely degraded 100 mg L−1 PHE within 36 h, showing improved degradation rate compared to their monocultures. In the co-culture, strain WY10 played a predominant role in PHE degradation. 1-hydroxy-2-naphthoic acid was an end-product of PHE degradation by strain WB9 and accumulated in the culture medium to serve as a substrate for strain WY10 growth, thereby accelerating PHE degradation. In turn, strain WY10 degraded PHE and 1-hydroxy-2-naphthoic acid intracellularly to form phthalate and protocatechuate that were exported to the culture medium through efflux transporters. However, strain WY10 cannot take up extracellular phthalate due to the absence of phthalate transporters, restricting phthalate degradation and PHE mineralization. In the co-culture, phthalate and protocatechuate accumulated in the culture medium were taken up and degraded towards TCA cycle by strain WB9. Therefore, the metabolic cross-feeding of strains WB9 and WY10 accelerated PHE degradation and mineralization. These findings exhibiting the synergistic degradation of PHE in the bacterial co-culture will facilitate its bioremediation application.
KW - Metabolic cross-feeding
KW - Phenanthrene
KW - Synergistic degradation
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U2 - 10.1016/j.jhazmat.2020.123825
DO - 10.1016/j.jhazmat.2020.123825
M3 - Article
C2 - 33264917
AN - SCOPUS:85091090779
SN - 0304-3894
VL - 403
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 123825
ER -