Abstract
Although eukaryotic messenger RNAs (mRNAs) normally possess a 5′ end N7-methyl guanosine (m7G) cap, a non-canonical 5′ nicotinamide adenine dinucleotide (NAD+) cap can tag certain transcripts for degradation mediated by the NAD+ decapping enzyme DXO1. Despite this importance, whether NAD+ capping dynamically responds to specific stimuli to regulate eukaryotic transcriptomes remains unknown. Here, we reveal a link between NAD+ capping and tissue- and hormone response-specific mRNA stability. In the absence of DXO1 function, transcripts displaying a high proportion of NAD+ capping are instead processed into RNA-dependent RNA polymerase 6-dependent small RNAs, resulting in their continued turnover likely to free the NAD+ molecules. Additionally, the NAD+-capped transcriptome is significantly remodeled in response to the essential plant hormone abscisic acid in a mechanism that is primarily independent of DXO1. Overall, our findings reveal a previously uncharacterized and essential role of NAD+ capping in dynamically regulating transcript stability during specific physiological responses.
Original language | English (US) |
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Pages (from-to) | 125-140.e6 |
Journal | Developmental Cell |
Volume | 56 |
Issue number | 1 |
DOIs | |
State | Published - Jan 11 2021 |
Keywords
- ABA
- NAD capping
- RNA degradation
- RNA modification
- RNA stability
- abscisic acid
- epitranscriptome
- post-transcriptional regulation
ASJC Scopus subject areas
- Molecular Biology
- General Biochemistry, Genetics and Molecular Biology
- Developmental Biology
- Cell Biology