Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy

Janine Mauzeroll; Allen J. Bard; Omeed Owhadian; Terrence J. Monks

doi:10.1073/pnas.0407613101

Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy

Janine Mauzeroll, Allen J. Bard, Omeed Owhadian, Terrence J. Monks

Pharmacology and Toxicology

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

The cytotoxicity of menadione on hepatocytes was studied by using the substrate generation/tip collection mode of scanning electrochemical microscopy by exposing the cells to menadione and detecting the menadione-S-glutathione conjugate (thiodione) that is formed during the cellular detoxication process and is exported from the cell by an ATP-dependent pump. This efflux was electrochemically detected and allowed scanning electrochemical microscopy monitoring and imaging of single cells and groups of highly confluent live cells. Based on a constant flux model, ≈6 × 10⁶ molecules of thiodione per cell per second are exported from monolayer cultures of Hep G2 cells.

Original language	English (US)
Pages (from-to)	17582-17587
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	101
Issue number	51
DOIs	https://doi.org/10.1073/pnas.0407613101
State	Published - Dec 21 2004

Keywords

Electrochemistry
Glutathione
Oxidative stress
SECM
Ultramicroelectrode

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.0407613101

Cite this

@article{34d71ae562fb4827a3c99c8dcc6ac702,

title = "Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy",

abstract = "The cytotoxicity of menadione on hepatocytes was studied by using the substrate generation/tip collection mode of scanning electrochemical microscopy by exposing the cells to menadione and detecting the menadione-S-glutathione conjugate (thiodione) that is formed during the cellular detoxication process and is exported from the cell by an ATP-dependent pump. This efflux was electrochemically detected and allowed scanning electrochemical microscopy monitoring and imaging of single cells and groups of highly confluent live cells. Based on a constant flux model, ≈6 × 106 molecules of thiodione per cell per second are exported from monolayer cultures of Hep G2 cells.",

keywords = "Electrochemistry, Glutathione, Oxidative stress, SECM, Ultramicroelectrode",

author = "Janine Mauzeroll and Bard, {Allen J.} and Omeed Owhadian and Monks, {Terrence J.}",

year = "2004",

month = dec,

day = "21",

doi = "10.1073/pnas.0407613101",

language = "English (US)",

volume = "101",

pages = "17582--17587",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "51",

}

TY - JOUR

T1 - Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy

AU - Mauzeroll, Janine

AU - Bard, Allen J.

AU - Owhadian, Omeed

AU - Monks, Terrence J.

PY - 2004/12/21

Y1 - 2004/12/21

N2 - The cytotoxicity of menadione on hepatocytes was studied by using the substrate generation/tip collection mode of scanning electrochemical microscopy by exposing the cells to menadione and detecting the menadione-S-glutathione conjugate (thiodione) that is formed during the cellular detoxication process and is exported from the cell by an ATP-dependent pump. This efflux was electrochemically detected and allowed scanning electrochemical microscopy monitoring and imaging of single cells and groups of highly confluent live cells. Based on a constant flux model, ≈6 × 106 molecules of thiodione per cell per second are exported from monolayer cultures of Hep G2 cells.

AB - The cytotoxicity of menadione on hepatocytes was studied by using the substrate generation/tip collection mode of scanning electrochemical microscopy by exposing the cells to menadione and detecting the menadione-S-glutathione conjugate (thiodione) that is formed during the cellular detoxication process and is exported from the cell by an ATP-dependent pump. This efflux was electrochemically detected and allowed scanning electrochemical microscopy monitoring and imaging of single cells and groups of highly confluent live cells. Based on a constant flux model, ≈6 × 106 molecules of thiodione per cell per second are exported from monolayer cultures of Hep G2 cells.

KW - Electrochemistry

KW - Glutathione

KW - Oxidative stress

KW - SECM

KW - Ultramicroelectrode

UR - http://www.scopus.com/inward/record.url?scp=11144243646&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144243646&partnerID=8YFLogxK

U2 - 10.1073/pnas.0407613101

DO - 10.1073/pnas.0407613101

M3 - Article

C2 - 15601769

AN - SCOPUS:11144243646

SN - 0027-8424

VL - 101

SP - 17582

EP - 17587

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 51

ER -

Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this