Abstract
The three-dimensional X-ray crystal structures of HincII bound to cognate DNA containing GTCGAC and Mn2+ or Mg2+, at 2.50 Å and 2.95 Å resolution, respectively, are presented. In both structures, the DNA is found cleaved, and the positions of the active-site groups, cleaved phosphate group, and 3′ oxygen atom of the leaving group are in very similar positions. Two highly occupied Mn2+ positions are found in each active site of the four crystallographically independent subunit copies in the HincII/DNA/Mn2+ structure. The manganese ion closest to the previously identified single Ca2+ position of HincII is shifted 1.7 Å and has lost direct ligation to the active-site aspartate residue, Asp127. A Mn2+-ligated water molecule in a position analogous to that seen in the HincII/DNA/Ca2+ structure, and proposed to be the attacking nucleophile, is beyond hydrogen bonding distance from the active-site lysine residue, Lys129, but remains within hydrogen bonding distance from the proRp oxygen atom of the phosphate group 3′ to the scissile phosphate group. In addition, the position of the cleaved phosphate group is on the opposite side of the axis connecting the two metal ions relative to that found in the BamHI/product DNA/Mn2+ structure. Mechanistic implications are discussed, and a model for the two-metal-ion mechanism of DNA cleavage by HincII is proposed.
Original language | English (US) |
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Pages (from-to) | 833-849 |
Number of pages | 17 |
Journal | Journal of Molecular Biology |
Volume | 343 |
Issue number | 4 |
DOIs | |
State | Published - Oct 29 2004 |
Keywords
- metal ion catalysis
- phosphoryl transfer
- protein-nucleic acid recognition
- restriction enzyme
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology
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Dive into the research topics of 'Mechanistic insights from the structures of HincII bound to cognate DNA cleaved from addition of Mg2+ and Mn2+'. Together they form a unique fingerprint.Datasets
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HincII bound to cleaved, cognate DNA containing GTCGAC
Etzkorn, C. (Contributor) & Horton, N. C. (Contributor), Protein Data Bank (PDB), Sep 28 2004
DOI: 10.2210/pdb1XHU/pdb, https://www.wwpdb.org/pdb?id=pdb_00001xhu
Dataset
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HincII bound to cleaved cognate DNA GTCGAC and Mn2+
Etzkorn, C. (Contributor) & Horton, N. C. (Contributor), Protein Data Bank (PDB), Sep 28 2004
DOI: 10.2210/pdb1XHV/pdb, https://www.wwpdb.org/pdb?id=pdb_00001xhv
Dataset