Mechanisms and control of NO-2 inhibition of anaerobic ammonium oxidation (anammox)

Guangbin Li; Jose Maria Carvajal-Arroyo; Reyes Sierra-Alvarez; Jim A. Field

doi:10.2175/106143017X14839994523064

Mechanisms and control of NO^-₂ inhibition of anaerobic ammonium oxidation (anammox)

Guangbin Li, Jose Maria Carvajal-Arroyo, Reyes Sierra-Alvarez, Jim A. Field

Chemical and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Nitrite (NO^-₂), one of the main substrates in the anaerobic ammonium oxidation (anammox) process, has the potential to inhibit anammox bacteria. The sensitivity of anammox cells with different energy status to NO^-₂ was evaluated, and addition of nitrate (NO^-₃ inhibition on the basis of narK gene with the putative function of facilitating NO^-₃ / NO^-₂ antiporter. The results showed that the resistance of anammox bacteria to NO^-₂ inhibition follows the order: activecells > starved-cells > resting-cells > starved-/resting-cells. Anammmox resting cells have increasing tolerance to NO^-₂ in the pH range from 7.0 to 7.5. Dissipating the proton gradient by using carbonyl cyanide m-chlorophenyl hydrazine (CCCP) caused severe inhibition at all pH values including pH = 7.5. Addition of NO^-₃ enabled activity recovery of NO^-₂-inhibited anammox bacteria regardless of whether the proton gradient was disrupted or not, supporting the hypothesis of NO^-₃ - dependent detoxification via a secondary transport system.

Original language	English (US)
Pages (from-to)	330-336
Number of pages	7
Journal	Water Environment Research
Volume	89
Issue number	4
DOIs	https://doi.org/10.2175/106143017X14839994523064
State	Published - Apr 1 2017

Keywords

Detoxification
Inhibitory mechanisms
Nitrate
Nitrite
Nitrogen removal
Proton gradient

ASJC Scopus subject areas

Environmental Chemistry
Ecological Modeling
Water Science and Technology
Waste Management and Disposal
Pollution

Access to Document

10.2175/106143017X14839994523064

Cite this

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title = "Mechanisms and control of NO-2 inhibition of anaerobic ammonium oxidation (anammox)",

abstract = "Nitrite (NO-2), one of the main substrates in the anaerobic ammonium oxidation (anammox) process, has the potential to inhibit anammox bacteria. The sensitivity of anammox cells with different energy status to NO-2 was evaluated, and addition of nitrate (NO-3 inhibition on the basis of narK gene with the putative function of facilitating NO-3 / NO-2 antiporter. The results showed that the resistance of anammox bacteria to NO-2 inhibition follows the order: activecells > starved-cells > resting-cells > starved-/resting-cells. Anammmox resting cells have increasing tolerance to NO-2 in the pH range from 7.0 to 7.5. Dissipating the proton gradient by using carbonyl cyanide m-chlorophenyl hydrazine (CCCP) caused severe inhibition at all pH values including pH = 7.5. Addition of NO-3 enabled activity recovery of NO-2-inhibited anammox bacteria regardless of whether the proton gradient was disrupted or not, supporting the hypothesis of NO-3 - dependent detoxification via a secondary transport system.",

keywords = "Detoxification, Inhibitory mechanisms, Nitrate, Nitrite, Nitrogen removal, Proton gradient",

author = "Guangbin Li and Carvajal-Arroyo, {Jose Maria} and Reyes Sierra-Alvarez and Field, {Jim A.}",

note = "Funding Information: This work was supported by the University of Arizona Water Sustainability Program, and the National Science Foundation (Contract CBET-1234211).",

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T1 - Mechanisms and control of NO-2 inhibition of anaerobic ammonium oxidation (anammox)

AU - Li, Guangbin

AU - Carvajal-Arroyo, Jose Maria

AU - Sierra-Alvarez, Reyes

AU - Field, Jim A.

N1 - Funding Information: This work was supported by the University of Arizona Water Sustainability Program, and the National Science Foundation (Contract CBET-1234211).

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Nitrite (NO-2), one of the main substrates in the anaerobic ammonium oxidation (anammox) process, has the potential to inhibit anammox bacteria. The sensitivity of anammox cells with different energy status to NO-2 was evaluated, and addition of nitrate (NO-3 inhibition on the basis of narK gene with the putative function of facilitating NO-3 / NO-2 antiporter. The results showed that the resistance of anammox bacteria to NO-2 inhibition follows the order: activecells > starved-cells > resting-cells > starved-/resting-cells. Anammmox resting cells have increasing tolerance to NO-2 in the pH range from 7.0 to 7.5. Dissipating the proton gradient by using carbonyl cyanide m-chlorophenyl hydrazine (CCCP) caused severe inhibition at all pH values including pH = 7.5. Addition of NO-3 enabled activity recovery of NO-2-inhibited anammox bacteria regardless of whether the proton gradient was disrupted or not, supporting the hypothesis of NO-3 - dependent detoxification via a secondary transport system.

AB - Nitrite (NO-2), one of the main substrates in the anaerobic ammonium oxidation (anammox) process, has the potential to inhibit anammox bacteria. The sensitivity of anammox cells with different energy status to NO-2 was evaluated, and addition of nitrate (NO-3 inhibition on the basis of narK gene with the putative function of facilitating NO-3 / NO-2 antiporter. The results showed that the resistance of anammox bacteria to NO-2 inhibition follows the order: activecells > starved-cells > resting-cells > starved-/resting-cells. Anammmox resting cells have increasing tolerance to NO-2 in the pH range from 7.0 to 7.5. Dissipating the proton gradient by using carbonyl cyanide m-chlorophenyl hydrazine (CCCP) caused severe inhibition at all pH values including pH = 7.5. Addition of NO-3 enabled activity recovery of NO-2-inhibited anammox bacteria regardless of whether the proton gradient was disrupted or not, supporting the hypothesis of NO-3 - dependent detoxification via a secondary transport system.

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KW - Nitrite

KW - Nitrogen removal

KW - Proton gradient

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