Mechanism of Filamentation-Induced Allosteric Activation of the SgrAI Endonuclease

Smarajit Polley, Dmitry Lyumkis, Nancy C. Horton

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The 3.5-Å cryo-EM structure of the filament formed by the type II restriction endonuclease SgrAI bound to DNA described in Polley et al. reveals the relevance of indirect readout for enzyme activity and a mechanism for filament-induced activation of DNA cleavage.

Original languageEnglish (US)
Pages (from-to)1497-1507.e3
Issue number10
StatePublished - Oct 1 2019


  • DNA binding
  • DNA sequence specificity
  • allostery
  • cryo-EM
  • endonuclease
  • enzyme mechanism
  • filament-forming enzyme
  • indirect readout
  • protein filament
  • self-association

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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