Measuring variations in optical imaging markers in a glial cell-directed mouse model of human MEN1 syndrome

Suzann Duan, Ricky Sontz, Juanita L. Merchant, Travis W. Sawyer

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Gastrointestinal neuroendocrine tumors (GI-NETs) including gastric carcinoids and duodenal neuroendocrine tumors (DNETs), represent a growing class of cancer.1 There is a strong need for intraoperative localization to facilitate diagnosis and treatment of DNETs, particularly those related to the hereditary MEN1 syndrome. However, these demands are precluded by a lack of in vivo model systems that accurately recapitulate disease heterogeneity and progression. Optical imaging markers are commonly used diagnostically to probe early tissue changes that occur with the onset of cancer. Promising techniques include autofluorescence imaging (AFI), which probes intrinsic biochemistry and metabolic markers, and optical coherence tomography (OCT), which provides a robust microstructural reference. Both have demonstrated widespread promise for non-invasive disease screening, making them potential candidates for localization of DNETs. Here we apply AFI and OCT to a mouse model of human MEN1 syndrome to identify unique optical markers associated with neuroendocrine cell reprogramming. Using Cre-lox technology, we generated a glial cell-directed Men1 knockout mouse model that exhibits enhanced neuroendocrine cell differentiation in the stomach and duodenum. We measured variations in optical imaging markers using AFI and OCT images of transgenic and wild type mice. The transgenic lines exhibit significant fluctuations in optical imaging markers compared to wild type mice, both in the scope of AFI and OCT (p<0.01). These results suggest that AFI and OCT may further inform biological and metabolic changes associated with initial neuroendocrine cell reprogramming prefacing tumor formation. Further studies are needed to fully elucidate the significance of these optical markers in GI-NET pathogenesis.

Original languageEnglish (US)
Title of host publicationLabel-free Biomedical Imaging and Sensing (LBIS) 2022
EditorsNatan T. Shaked, Oliver Hayden
PublisherSPIE
ISBN (Electronic)9781510648159
DOIs
StatePublished - 2022
EventLabel-free Biomedical Imaging and Sensing (LBIS) 2022 - Virtual, Online
Duration: Feb 20 2022Feb 24 2022

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11972
ISSN (Print)1605-7422

Conference

ConferenceLabel-free Biomedical Imaging and Sensing (LBIS) 2022
CityVirtual, Online
Period2/20/222/24/22

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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