@inbook{854de6318a4e4c40a1d155aaf97509c8,
title = "Measuring FOXO Nuclear Shuttling Dynamics by Fluorescence Microscopy",
abstract = "FOXO transcription factors respond to a number of different stresses by shuttling from the cytoplasm to the nucleus where they upregulate hundreds of target genes with diverse cellular functions. The cellular consequences of FOXO activation are both stress and cell-type specific. Recent evidence suggests that one way in which FOXO dictates stress-specific outcomes is through distinct nuclear/cytoplasmic shuttling dynamics. Here we outline methods for measuring FOXO nuclear shuttling dynamics using fluorescence-based reporters.",
keywords = "Cell fate, FOXO, Immunofluorescence, Nuclear to cytoplasmic shuttling, Protein dynamics, Time-lapse microscopy",
author = "Elizabeth Jose and Paek, {Andrew L.}",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.",
year = "2025",
doi = "10.1007/978-1-0716-4217-7_12",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "131--143",
booktitle = "Methods in Molecular Biology",
}