Measuring Caenorhabditis elegans life span on solid media

George L. Sutphin, Matt Kaeberlein

Research output: Contribution to journalArticlepeer-review

116 Scopus citations

Abstract

Aging is a degenerative process characterized by a progressive deterioration of cellular components and organelles resulting in mortality. The nematode Caenorhabditis elegans has emerged as a principal model used to study the biology of aging. Because virtually every biological subsystem undergoes functional decline with increasing age, life span is the primary endpoint of interest when considering total rate of aging. In nematodes, life span is typically defined as the number of days an animal remains responsive to external stimuli. Nematodes can be propagated either in liquid media or on solid media in plates, and techniques have been developed for measuring life span under both conditions. Here we present a generalized protocol for measuring life span of nematodes maintained on solid nematode growth media and fed a diet of UV-killed bacteria. These procedures can easily be adapted to assay life span under various common conditions, including a diet consisting of live bacteria, dietary restriction, and RNA interference.

Original languageEnglish (US)
Article numbere1152
JournalJournal of Visualized Experiments
Issue number27
DOIs
StatePublished - May 2009
Externally publishedYes

Keywords

  • Aging
  • Caenorhabditis elegans
  • Developmental biology
  • Dietary restriction
  • Issue 27
  • Life span assay
  • Longevity
  • Nematode
  • RNA interference
  • Worms

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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