Maturation of intracellular calcium homeostasis in sheep pulmonary arterial smooth muscle cells

Ravi Goyal, Kara D. Creel, Erica Chavis, Gregory D. Smith, Lawrence D. Longo, Sean M. Wilson

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Cytosolic Ca2+ signaling dynamics are important to pulmonary arterial reactivity, and alterations are implicated in pulmonary vascular disorders. Yet, adaptations in cellular Ca2+ homeostasis and receptor-mediated Ca2+ signaling with maturation from fetal to adult life in pulmonary arterial smooth muscle cells (PASMCs) are not known. The present study tested the hypothesis that cytosolic Ca2+ homeostasis and receptor-generated Ca2+ signaling adapt with maturation in sheep PASMCs. Digitalized fluorescence microscopy was performed using isolated PASMCs from fetal and adult sheep that were loaded with the Ca2+ indicator fura 2. The results show that basal cytosolic and sarcoplasmic reticulum Ca 2+ levels are attained before birth. Similarly, Ca2+ efflux pathways from the cytosol and basal as well as capacitative Ca 2+ entry (CCE) are also developed before birth. However, receptor-mediated Ca2+ signaling adapts with maturation. Prominently, serotonin stimulation elicited Ca2+ elevations in very few fetal compared with adult PASMCs; in contrast, phenylephrine elevated Ca2+ in a similar percentage of fetal and adult PASMCs. Serotonin and phenylephrine elicited Ca2+ increases of a similar magnitude in reactive cells of fetus and adult, supporting the assertion that inositol trisphosphate signaling is intact. Caffeine and ATP elevated Ca2+ in equivalent numbers of fetal and adult PASMCs. However, the caffeine-induced cytosolic Ca2+ increase was significantly greater in fetal PASMCs, whereas the ATP-elicited increase was greater in adult cells. Overall, the results of this study demonstrate selective adaptations in receptor-mediated Ca2+ signaling, but not in cellular Ca2+ homeostasis.

Original languageEnglish (US)
Pages (from-to)L905-L914
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume295
Issue number5
DOIs
StatePublished - Nov 2008
Externally publishedYes

Keywords

  • ATP
  • Caffeine
  • Capacitative Ca entry
  • Fura 2
  • Inositol trisphosphate
  • Plasma membrane Ca-ATPase
  • Ryanodine receptor
  • Sarco(endo)plasmic reticulum Ca-ATPase
  • Serotonin

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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