TY - JOUR
T1 - Mathematical modeling of the batch adsorption of proteins on new restricted access media with poly(Ethylene glycol) as a semipermeable barrier using compact finite differences
AU - Gonzalez-Ortega, Omar
AU - Guzman, Roberto
PY - 2018/1/1
Y1 - 2018/1/1
N2 - In this work we describe a mathematical analysis of the batch adsorption process of several proteins using a new restricted access medium consisting of agarose beads grafted with poly(ethylene glycol) (PEG) as a semi-permeable barrier and immobilized metal ions or ion exchange groups as binding sites. The model was fitted to experimental data, allowing the estimation of the adsorption rate constant and the effective diffusivity for each protein. The model was solved using compact finite differences in a MATLAB® platform. According to the results, the presence of grafted PEG reduces the adsorption of all proteins to different extent; with high molecular weight proteins being affected the most. The model also establishes a reduction in the adsorption rate constant (which affects protein interaction with binding sites). The movement of the protein molecules in the adsorbent pores is also affected by the grafted PEG, but to a lesser extent.
AB - In this work we describe a mathematical analysis of the batch adsorption process of several proteins using a new restricted access medium consisting of agarose beads grafted with poly(ethylene glycol) (PEG) as a semi-permeable barrier and immobilized metal ions or ion exchange groups as binding sites. The model was fitted to experimental data, allowing the estimation of the adsorption rate constant and the effective diffusivity for each protein. The model was solved using compact finite differences in a MATLAB® platform. According to the results, the presence of grafted PEG reduces the adsorption of all proteins to different extent; with high molecular weight proteins being affected the most. The model also establishes a reduction in the adsorption rate constant (which affects protein interaction with binding sites). The movement of the protein molecules in the adsorbent pores is also affected by the grafted PEG, but to a lesser extent.
KW - Batch adsorption modeling
KW - Compact finite differences
KW - IEX
KW - IMAC
KW - Restricted access medium
UR - http://www.scopus.com/inward/record.url?scp=85046079858&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046079858&partnerID=8YFLogxK
U2 - 10.1590/0104-6632.20180351s20160404
DO - 10.1590/0104-6632.20180351s20160404
M3 - Article
AN - SCOPUS:85046079858
VL - 35
SP - 237
EP - 251
JO - Brazilian Journal of Chemical Engineering
JF - Brazilian Journal of Chemical Engineering
SN - 0104-6632
IS - 1
ER -