Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal α2→3 and α2→6 sialogalactosylated linkages at subpicomole levels

Peng Chen; Ulrike Werner-Zwansiger; Donald Wiesler; Marty Pagel; Milos V. Novotny

doi:10.1021/ac990674w

Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal α2→3 and α2→6 sialogalactosylated linkages at subpicomole levels

Peng Chen
, Ulrike Werner-Zwansiger
, Donald Wiesler
, Marty Pagel
, Milos V. Novotny

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Perbenzoylated sialooligosaccharides were found to be stable derivatives, giving intense signals during the matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis in the positive-ion mode. Terminal Neu5NAcα2→3 and α2→6Gal units of oligosaccharides undergo characteristic structural changes during benzoylation, yielding easily recognizable mass spectral patterns. Subpicomole carbohydrate samples were successfully benzoylated and analyzed through MALDI mass spectrometry.

Original language	English (US)
Pages (from-to)	4969-4973
Number of pages	5
Journal	Analytical Chemistry
Volume	71
Issue number	21
DOIs	https://doi.org/10.1021/ac990674w
State	Published - Nov 1 1999
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

Access to Document

10.1021/ac990674w

Cite this

@article{9fe180d1f88f4110a1be927ac444e3ef,

title = "Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal α2→3 and α2→6 sialogalactosylated linkages at subpicomole levels",

abstract = "Perbenzoylated sialooligosaccharides were found to be stable derivatives, giving intense signals during the matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis in the positive-ion mode. Terminal Neu5NAcα2→3 and α2→6Gal units of oligosaccharides undergo characteristic structural changes during benzoylation, yielding easily recognizable mass spectral patterns. Subpicomole carbohydrate samples were successfully benzoylated and analyzed through MALDI mass spectrometry.",

author = "Peng Chen and Ulrike Werner-Zwansiger and Donald Wiesler and Marty Pagel and Novotny, \{Milos V.\}",

year = "1999",

month = nov,

day = "1",

doi = "10.1021/ac990674w",

language = "English (US)",

volume = "71",

pages = "4969--4973",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "21",

}

TY - JOUR

T1 - Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal α2→3 and α2→6 sialogalactosylated linkages at subpicomole levels

AU - Chen, Peng

AU - Werner-Zwansiger, Ulrike

AU - Wiesler, Donald

AU - Pagel, Marty

AU - Novotny, Milos V.

PY - 1999/11/1

Y1 - 1999/11/1

N2 - Perbenzoylated sialooligosaccharides were found to be stable derivatives, giving intense signals during the matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis in the positive-ion mode. Terminal Neu5NAcα2→3 and α2→6Gal units of oligosaccharides undergo characteristic structural changes during benzoylation, yielding easily recognizable mass spectral patterns. Subpicomole carbohydrate samples were successfully benzoylated and analyzed through MALDI mass spectrometry.

AB - Perbenzoylated sialooligosaccharides were found to be stable derivatives, giving intense signals during the matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis in the positive-ion mode. Terminal Neu5NAcα2→3 and α2→6Gal units of oligosaccharides undergo characteristic structural changes during benzoylation, yielding easily recognizable mass spectral patterns. Subpicomole carbohydrate samples were successfully benzoylated and analyzed through MALDI mass spectrometry.

UR - https://www.scopus.com/pages/publications/0033230276

UR - https://www.scopus.com/pages/publications/0033230276#tab=citedBy

U2 - 10.1021/ac990674w

DO - 10.1021/ac990674w

M3 - Article

C2 - 10565286

AN - SCOPUS:0033230276

SN - 0003-2700

VL - 71

SP - 4969

EP - 4973

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 21

ER -

Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal α2→3 and α2→6 sialogalactosylated linkages at subpicomole levels

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this