TY - GEN
T1 - Mapping the micromechanical properties of cryo-sectioned aortic tissue with scanning acoustic microscopy
AU - Akhtar, Riaz
AU - Sherratt, Michael J.
AU - Watson, Rachel E.B.
AU - Kundu, Tribikram
AU - Derby, Brian
PY - 2008
Y1 - 2008
N2 - Although the gross mechanical properties of ageing tissues have been extensively documented, biological tissues are highly heterogeneous and little is known concerning the variation of micro-mechanical properties within tissues. Here, we use Scanning Acoustic Microscopy (SAM) to map the acoustic wave speed (a measure of stiffness) as a function of distance from the outer adventitial layer of cryo-sectioned ferret aorta. With a 400 MHz lens, the images of the aorta samples matched those obtained following chemical fixation and staining of sections which were viewed with fluorescence microscopy. Quantitative analysis was conducted with a frequency scanning or V(f) technique by imaging the tissue from 960 MHz to 1.1 GHz. Undulating acoustic wave speed (stiffness) distributions corresponded with elastic fibre locations in the tissue; there was a decrease in wave speed of around 40 ms-1 from the adventitia (outer layer) to the intima (innermost).
AB - Although the gross mechanical properties of ageing tissues have been extensively documented, biological tissues are highly heterogeneous and little is known concerning the variation of micro-mechanical properties within tissues. Here, we use Scanning Acoustic Microscopy (SAM) to map the acoustic wave speed (a measure of stiffness) as a function of distance from the outer adventitial layer of cryo-sectioned ferret aorta. With a 400 MHz lens, the images of the aorta samples matched those obtained following chemical fixation and staining of sections which were viewed with fluorescence microscopy. Quantitative analysis was conducted with a frequency scanning or V(f) technique by imaging the tissue from 960 MHz to 1.1 GHz. Undulating acoustic wave speed (stiffness) distributions corresponded with elastic fibre locations in the tissue; there was a decrease in wave speed of around 40 ms-1 from the adventitia (outer layer) to the intima (innermost).
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U2 - 10.1557/proc-1132-z03-07
DO - 10.1557/proc-1132-z03-07
M3 - Conference contribution
AN - SCOPUS:77950480702
SN - 9781615677665
T3 - Materials Research Society Symposium Proceedings
SP - 41
EP - 46
BT - Mechanics of Biological and Biomedical Materials
PB - Materials Research Society
T2 - 2008 MRS Fall Meeting
Y2 - 1 December 2008 through 5 December 2008
ER -