Mapping of the gene for hormone sensitive lipase (LIPE) to chromosome 19q13.1 → q13.2

R. C. Levitt; Z. Liu; N. Nouri; D. A. Meyers; B. Brandriff; H. M. Mohrenweiser

Mapping of the gene for hormone sensitive lipase (LIPE) to chromosome 19q13.1 → q13.2

R. C. Levitt, Z. Liu, N. Nouri, D. A. Meyers, B. Brandriff, H. M. Mohrenweiser

Research output: Contribution to journal › Article › peer-review

Abstract

Free fatty acids are the major source of fuel for mammals, and hormone sensitive lipase (LIPE) plays a critical role in lipid metabolism by mobilizing free fatty acids from stored triglycerides. We have identified and sequenced a partial cDNA for LIPE. Cosmids were identified by hybridization and mapped to 19q13.1 → q13.2 by FISH. Direct sequence analysis of a 1 kb segment of cosmid 26710 identifies a dinucleotide repeat in an intron upstream of exon 8 of human LIPE. This marker was heterozygous 82 % of the time with 12 alleles (166-190 bp) detected in 122 chromosomes. The most likely order for this gene is: qter-[D19S178/LIPE]-(3 cM)-D19S47-(1 cM)-D19S190-RYR1-cen.

Original language	English (US)
Pages (from-to)	211-214
Number of pages	4
Journal	Cytogenetics and Cell Genetics
Volume	69
Issue number	3-4
State	Published - 1995
Externally published	Yes

ASJC Scopus subject areas

Genetics
Cell Biology

Cite this

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title = "Mapping of the gene for hormone sensitive lipase (LIPE) to chromosome 19q13.1 → q13.2",

abstract = "Free fatty acids are the major source of fuel for mammals, and hormone sensitive lipase (LIPE) plays a critical role in lipid metabolism by mobilizing free fatty acids from stored triglycerides. We have identified and sequenced a partial cDNA for LIPE. Cosmids were identified by hybridization and mapped to 19q13.1 → q13.2 by FISH. Direct sequence analysis of a 1 kb segment of cosmid 26710 identifies a dinucleotide repeat in an intron upstream of exon 8 of human LIPE. This marker was heterozygous 82 % of the time with 12 alleles (166-190 bp) detected in 122 chromosomes. The most likely order for this gene is: qter-[D19S178/LIPE]-(3 cM)-D19S47-(1 cM)-D19S190-RYR1-cen.",

author = "Levitt, {R. C.} and Z. Liu and N. Nouri and Meyers, {D. A.} and B. Brandriff and Mohrenweiser, {H. M.}",

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T1 - Mapping of the gene for hormone sensitive lipase (LIPE) to chromosome 19q13.1 → q13.2

AU - Levitt, R. C.

AU - Liu, Z.

AU - Nouri, N.

AU - Meyers, D. A.

AU - Brandriff, B.

AU - Mohrenweiser, H. M.

PY - 1995

Y1 - 1995

N2 - Free fatty acids are the major source of fuel for mammals, and hormone sensitive lipase (LIPE) plays a critical role in lipid metabolism by mobilizing free fatty acids from stored triglycerides. We have identified and sequenced a partial cDNA for LIPE. Cosmids were identified by hybridization and mapped to 19q13.1 → q13.2 by FISH. Direct sequence analysis of a 1 kb segment of cosmid 26710 identifies a dinucleotide repeat in an intron upstream of exon 8 of human LIPE. This marker was heterozygous 82 % of the time with 12 alleles (166-190 bp) detected in 122 chromosomes. The most likely order for this gene is: qter-[D19S178/LIPE]-(3 cM)-D19S47-(1 cM)-D19S190-RYR1-cen.

AB - Free fatty acids are the major source of fuel for mammals, and hormone sensitive lipase (LIPE) plays a critical role in lipid metabolism by mobilizing free fatty acids from stored triglycerides. We have identified and sequenced a partial cDNA for LIPE. Cosmids were identified by hybridization and mapped to 19q13.1 → q13.2 by FISH. Direct sequence analysis of a 1 kb segment of cosmid 26710 identifies a dinucleotide repeat in an intron upstream of exon 8 of human LIPE. This marker was heterozygous 82 % of the time with 12 alleles (166-190 bp) detected in 122 chromosomes. The most likely order for this gene is: qter-[D19S178/LIPE]-(3 cM)-D19S47-(1 cM)-D19S190-RYR1-cen.

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Mapping of the gene for hormone sensitive lipase (LIPE) to chromosome 19q13.1 → q13.2

Abstract

ASJC Scopus subject areas

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