Abstract
Comparing the DNA sequence of maize genes and chromosomal regions to their maize homeologs and to other grass orthologs provides a quick check for many annotation errors and may identify useful regions of conservation and divergence. Fractionation and subfunctionalization, two processes that shape the evolution of genes and genomes, may be evaluated by comparing syntenic chromosomal regions within and among genomes. We provide an overview of maize's genome evolution, emphasizing the processes of fractionation and subfunctionalization. We then provide a tutorial of our software for multiple DNA sequence alignment display and analysis. As an example, we begin with one maize gene sequence, find a pair of maize home-ologous gene regions, and add an orthologous segment from sorghum and rice; we then analyze this 4-way DNA alignment.
Original language | English (US) |
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Title of host publication | Handbook of Maize |
Subtitle of host publication | Genetics and Genomics |
Publisher | Springer New York |
Pages | 341-351 |
Number of pages | 11 |
ISBN (Electronic) | 9780387778631 |
ISBN (Print) | 9780387778624 |
DOIs | |
State | Published - 2009 |
Externally published | Yes |
ASJC Scopus subject areas
- General Agricultural and Biological Sciences
- General Biochemistry, Genetics and Molecular Biology